【摘要】 采用壳聚糖作为载体，加入牡蛎壳羟基磷灰石制备水凝胶。将鲎血浆粉脱铜、水解获得蛋白肽，掺入水凝胶中获得鲎血浆蛋白水凝胶。测试水凝胶中蛋白肽释放率、吸水率、降解率，并用热重分析和SEM对水凝胶进行表征。当水凝胶实验配方为(质量分数)2.1%的壳聚糖、5.5%的β-甘油磷酸钠、2.5%的羟基磷灰石、0.12%鲎血蛋白时，鲎血蛋白水凝胶的蛋白释放率达71.44%、吸水率达432.28%、体外降解率为50.04%。鲎血蛋白水凝胶具有温敏性，蛋白释放率好，吸水率高。鲎血蛋白水凝胶可应用于骨修复材料。更多还原

【Abstract】 Hydroxyapatite from oyster shell was added to chitosan as a carrier to prepare hydrogel, and then incorporating the copper-removal protein peptide after hydrolysis of limulus plasma to obtain the limulus plasma-protein hydrogel. And then its releasing rate of protein peptide, water absorption rate and biodegradation rate were tested. Finally, the hydrogel was characterized by the thermogravimetric analysis and SEM. When the experimental formula of hydrogel comprised 2.1% of chitosan, 5.5% of sodium β-glycerophosphate, 2.5% of hydroxyapatite, 0.12% of the limulus copper-removal protein peptide, the releasing rate of protein peptide reached 71.44%, the water absorption rate was 432.28%, and the in vitro biodegradation rate was 50.04%. Limulus plasma-protein hydrogel displayed the temperature sensitivity, the better releasing rate of protein peptide and the high water absorption rate. Limulus plasma-protein hydrogel could be used in the bone repair materials.更多还原