【摘要】 螯合剂能增加植物对重金属的吸收和积累，但在大麻上的研究报道十分鲜见.采用盆栽的方法，以巴马火麻为试验材料，在商用泥炭基质中添加2 000 mg·kg-1的重金属铅，研究了不同浓度（0、2、5、10 mmol·L-1和15 mmol·L-1）螯合剂乙二胺四乙酸二钠（EDTA）与柠檬酸（CA）对基质中铅活化和对大麻生长、铅吸收与积累的影响.结果显示，基质有效态铅含量随螯合剂浓度增加而增加，在15 mmol·L-1时达到最大；大麻的生长如株高、茎粗和生物量在添加2 mmol·L-1 EDTA和2～5 mmol·L-1 CA时略有增加，其他处理均使大麻生长指标有不同程度的降低；在5 mmol·L-1 EDTA处理下，大麻根、茎、叶的铅质量比达最大值，分别为428.75、307.68 mg·kg-1和287.44 mg·kg-1，显著高于对照（0）.大麻铅转运系数在2 mmol·L-1 EDTA处理下最高，是对照的1.79倍.由此可见，适当施加螯合剂能够有效地活化基质中的铅并促进大麻对铅的吸收、积累与转运，添加5 mmol·L-1 EDTA和10 mmol·L-1 CA时大麻对铅污染基质的修复效果最佳.更多还原

【Abstract】 Chelators has been known to increase heavy metal absorption and accumulation in some plants, but their efficacy in hemp is very seldom realized. Using pot culture, a local hemp variety Bamahuoma was used as experimental material, and 2 000 mg·kg-1 heavy metal lead(Pb) was added to the commercial peat substrate. The effects of chelators disodium ethylene diamine tetraacetic acid(EDTA) and citric acid(CA) with different concentrations(0, 2, 5, 10 mmol·L-1 and 15 mmol·L-1) on Pb activation in the substrate and on the growth, Pb absorption and accumulation of hemp were studied in this paper. The results showed that the content of available Pb in substrate increased with the increase of chelator concentration, and it reached the maximum at 15 mmol·L-1.The growth of hemp, such as plant height, stem diameter and biomass, increased slightly when 2 mmol·L-1 EDTA and 2～5 mmol·L-1 CA was added, respectively, and the growth indexes of hemp decreased in varying degrees in other treatments. Under the treatment of 5 mmol·L-1 EDTA, Pb contents in hemp root, stem and leaf reached the maximum, showing 428.75, 307.68 and 287.44 mg·kg-1, respectively, which was significantly higher than that of the control(0 mmol·L-1). Hemp had the highest Pb transport coefficient under 2 mmol·L-1 EDTA treatment, 1.79times of the control. The results suggested that appropriate amount of chelator could effectively activate Pb in the substrate and promote the absorption, accumulation and transport of Pb by hemp; hemp showed the best remediation efficacy, when 5 mmol·L-1 EDTA or 10 mmol·L-1 CA was added in Pb contaminated substrate.更多还原