【摘要】 目的 基于网络药理学和GEO数据研究中药三七、黄芪、丹参防治缺血性脑卒中作用机制。方法 通过TCMSP数据库筛选得到三七、黄芪、丹参中活性成分以及潜在靶点，在OMIM和Genecards数据库中检索获取缺血性脑卒中疾病相关靶点，用Cytoscape软件构建“中药-活性成分-作用靶点”以及“疾病-中药-活性成分-作用靶点”网络图。将三七、黄芪、丹参有效成分靶点与缺血性脑卒中（ischemic stroke,IS）疾病靶点交集后所得相关靶点蛋白通过STRING平台以及Cytoscape软件构建蛋白-蛋白相互作用（PPI）网络和潜在核心靶蛋白。利用DAVID平台对潜在核心靶蛋白进行GO富集分析和KEGG通路富集分析。同时结合GEO数据集对核心靶蛋白进行验证。结果 中药三七中活性成分为13个，黄芪活性成分为21个，丹参活性成分为65个。三七、黄芪、丹参活性成分在IS中的潜在作用靶蛋白160个，其中筛选得到潜在核心靶蛋白8个。核心蛋白在GO富集和KFGG通路富集中涉及脂多糖介导的信号通路、RNA聚合酶Ⅱ启动子对转录的正调控蛋白磷酸酶、MAPK信号通路、T细胞受体信号通路、Toll样受体信号通路等。其中JUN和TNF为关键核心靶蛋白。结论 三七、黄芪、丹参在缺血性脑卒中可以通过多靶点、多途径发挥作用，MAPK和Toll样受体等多信号通路，JUN和TNF等多作用靶点是三七、黄芪、丹参多活性成分治疗缺血性脑卒中的作用机制。更多还原

【Abstract】 Objective To study the mechanism of Panax notoginseng,Radix Astragali and Salvia miltiorrhiza in preventing and treating ischemic stroke(IS),on the basis of network pharmacology and GEO database.Methods The active components and potential targets of Panax notoginseng,Radix Astragali and Salvia miltiorrhiza were screened from TCMSP(Traditional Chinese Medicine Systems Pharmacology)Database.Targets related to IS were obtained from OMIM and Genecards Database.The software Cytoscape was adopted to draw the network diagrams of“Chinese medicines-active components-target”and “disease-Chinese medicinesactive components-target”.After the targets of active components of Panax notoginseng,Radix Astragali and Salvia miltiorrhiza were intersected with the disease targets of IS,the related target proteins were treated on STRING platform and Cytoscape to construct protein-protein interaction(PPI)network and potential core target proteins.GO(Gene ontology)enrichment analysis and KEGG pathway enrichment analysis were conducted for the potential core target proteins by using DAVID platform.At the same time,the core target protein was verified with GEO data set． Results There were 13active components in Panax notoginseng,21in Radix Astragali and 65in Salvia miltiorrhiza.There were 160potential IS-related target proteins in the active components of Panax notoginseng,Radix Astragali and Salvia miltiorrhiza,among which 8core target proteins were screened out.The results of Core protein enrichment in GO and KFGG pathway involved lipopolysaccharidemediated signaling pathway,positive regulation of transcription from RNA polymerase II promoter,MAPK signaling pathway,T cell receptor signaling pathway,Toll-like receptor signaling pathway,TNF signaling pathway and so on.Among them,JUN and TNF were the key core target proteins． Conclusion Panax notoginseng,Radix Astragali and Salvia miltiorrhiza can play a role in ischemic stroke through multiple targets and pathways.Multiple signal pathways such as MAPK and Toll-like receptors,as well as multiple targets such as JUN and TNF are the working mechanism of multiple active components of Panax notoginseng,Radix Astragali and Salvia miltiorrhiza in the treatment of ischemic stroke.更多还原