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草莓自毒物质对香豆酸降解菌的筛选及其降解效果研究

Study on the Screening Degrading Bacteria and Degradation Effect P-coumarac Acid in Strawberry Autotoxin

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【作者】 刘紫英杜贵勇傅苒馨刘小林

【Author】 LIU Zi-ying;DU Gui-yong;FU Ran-xin;LIU Xiao-lin;College of Chemistry and Bioengineering,Yichun University;Wanzai County Bureau of Agriculture and Rural Affairs;College of Life Science and Resources Environment,Yichun University;

【通讯作者】 刘小林;

【机构】 宜春学院化学与生物工程学院万载县农业农村局宜春学院生命科学与资源环境学院

【摘要】 目的:对香豆酸是相对分布较广的一类酚酸类化感自毒物质,本文以其筛选1株对自毒物质对香豆酸降解效果较好的细菌。方法:以对香豆酸为唯一碳源,经富集分离得到高效降解菌,采用分光光度法对其解毒能力进行探究,并对分离所得菌株在进行形态学鉴定的基础上并进一步进行16SrRNA分子鉴定。结果:筛选得到7株对对香豆酸具有解毒能力的菌株,其中菌株DXDS515在72 h时降解率最高,达到59.27%,经形态鉴定和分子鉴定为霍氏肠杆菌属。结论:筛选的降解菌DXDS515可降解草莓自毒物质对香豆酸。

【Abstract】 Objective:P-coumaric acid belongs to strawberry autotoxin, which is a relatively widely distributed phenolic allelochemical.In this study we in order to screen out a strain of bacterium which was better at degradation of p-coumaric acid.Method:P-coumaric acid was the sole carbon source, using spectrophotometry to explore its detoxification ability, and the isolated strains were preliminarily identified and further analyzed by 16SrRNA molecular identification.Result:Seven strains with the ability to detoxify p-coumaric acid were screened, and the degradation rate of the strain DXDS515 reached 59.27% at 72 hours.After microscopic identification and molecular identification, it was identified as Enterobacter sp.Conclusion:The bacterium DXDS515 screened can degrade p-coumaric acid.

【基金】 国家自然科学基金项目(编号:31660603);江西省教育厅2021年度科技计划项目(编号:GJJ211625)
  • 【文献出处】 宜春学院学报 ,Journal of Yichun University , 编辑部邮箱 ,2022年12期
  • 【分类号】S182
  • 【下载频次】74
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