【摘要】 目的 探讨肝细胞癌（肝癌）组织中连接蛋白Cx32、磷酸化Src(p-Src)Y416、总Src(total Src)、磷酸化局部黏着斑激素（p-FAK）Y925、总FAK(total FAK)蛋白表达水平对肝癌术后患者预后的影响。方法 收集97例接受根治性手术肝癌患者的肝癌组织标本和相应的癌旁组织标本，使用免疫组织化学（免疫组化）染色法测定Cx32、p-Src Y416、total Src、p-FAK Y925和total FAK的蛋白表达水平，并对肝癌患者术后随访5年，采用单因素和多因素Cox回归分析肝癌患者术后死亡的影响因素，并使用Kaplan-Meier法计算生存率。结果 免疫组化染色结果显示肝癌组织中Cx32的阳性表达率低于相应的癌旁组织，而p-Src Y416、total Src、p-FAK Y925、total FAK的蛋白阳性表达率均高于相应的癌旁组织（P均<0.05）。单因素Cox回归分析显示，年龄、肿瘤分化程度、肿瘤直径、Cx32、p-Src、total Src、p-FAK Y925、total FAK是肝癌患者术后死亡的8个可疑影响因素（P均<0.05）；多因素Cox回归分析及生存分析显示，高表达Cx32可能是肝癌患者术后死亡风险的独立保护因素，而高表达p-Src Y416和p-FAK Y925可能是肝癌患者术后死亡风险的独立危险因素（P均<0.05）。结论 高表达Cx32提示肝癌患者术后死亡风险可能较低，而高表达p-Src Y416和p-FAK Y925提示肝癌患者术后死亡风险较高。更多还原

【Abstract】 Objective To determine the protein expression levels of Connexin32(Cx32),phosphorylated-Src(p-Src) Y416,total Src,phosphorylated-FAK(p-FAK) Y925 and total FAK in the hepatocellular carcinoma(HCC) tissues,and to evaluate the effect of these proteins on prognosis of HCC patients after hepatectomy.Methods HCC and corresponding paracancerous tissues of patients who underwent radical operation from January 2014 to December 2014 were collected.The protein expression levels of Cx32,p-Src Y416,total Src,p-FAK Y925 and total FAK were measured by immunohistochemistry.Postoperatively,HCC patients were followed up for 5 years.Univarivate and multivariate Cox’s regression analyses were employed to identify the influencing factors of postoperative mortality,and kaplan-Meier curve was delineated to evaluate the survival rate.Results Immunohistochemistry showed that the positive protein expression rate of Cx32 in the HCC tissues was significantly lower,whereas the positive protein expression rates of p-Src Y416,total Src,p-FAK Y925 and total FAK were significantly higher compared with those in the corresponding paracancerous tissues(all P <0.05).Univarivate Cox’s regression analysis indicated that age,degree of tumor differentiation,tumor diameter,Cx32,p-Src Y416,total Src,p-FAK Y925 and total FAK were eight suspected influencing factors of postoperative mortality of HCC patients(all P < 0.05).Multivariate Cox’s regression analysis and survival analysis showed that Cx32 protein expression could be an independent protective factor of postoperative mortality for HCC patients,whereas high protein expression levels of p-Src Y416 and p-FAK Y925 were the independent risk factors(all P < 0.05).Conclusion Cx32 expression reduces the mortality risk of HCC patients after radical operation,while high expression levels of p-Src Y416 and p-FAK Y925 enhance postoperative mortality risk.更多还原