【摘要】 目的 探讨整合素连接激酶(ILK)和黏着斑激酶(FAK)在宫颈鳞状细胞癌(CSCC)组织中的表达及其与CSCC临床病理特征、人乳头状瘤病毒(HPV)感染的关系。方法 选择2015年10月至2019年8月邯郸市第一医院保存的30例正常宫颈组织、30例宫颈上皮内瘤变(CIN)组织和60例CSCC组织标本，采用实时荧光定量聚合酶链式反应检测正常宫颈组织、CIN组织、CSCC组织中ILK和FAK mRNA的表达，采用免疫组织化学法检测正常宫颈组织、CIN组织、CSCC组织中ILK和FAK蛋白的表达，并分析ILK和FAK蛋白表达与CSCC患者临床病理特征及HPV感染的关系。结果 CSCC组织中ILK、FAK mRNA的相对表达量分别为12.37±1.26、17.32±3.21,CIN组织中ILK、FAK mRNA的相对表达量分别为8.39±3.20、13.35±2.34,正常宫颈组织中ILK、FAK mRNA的相对表达量分别为3.67±2.40、5.47±2.10;CIN组织和CSCC组织中ILK、FAK mRNA的相对表达量显著高于正常宫颈组织(P<0.05),CSCC组织中ILK、FAK mRNA的相对表达量显著高于CIN组织(P<0.05)。正常宫颈组织中ILK、FAK蛋白阳性表达率分别为6.67%(2/30)、3.33%(1/30),CIN组织中ILK、FAK蛋白阳性表达率分别为63.33%(19/30)、56.67%(17/30),CSCC组织中ILK、FAK蛋白阳性表达率分别为85.00%(51/60)、71.67%(43/60);CSCC组织中ILK、FAK蛋白阳性表达率显著高于CIN组织和正常宫颈组织(P<0.05),CIN组织中ILK、FAK蛋白阳性表达率显著高于正常宫颈组织(P<0.05)。ILK和FAK蛋白表达与CSCC的临床分期、淋巴结转移有关(P<0.05),而ILK和FAK蛋白表达与CSCC患者年龄无关(P>0.05)。CSCC和CIN组织中HPV-DNA阳性75例，HPV-DNA阴性15例。HPV-DNA阳性者ILK蛋白阳性表达率为86.67%(65/75),HPV-DNA阴性者ILK蛋白阳性表达率为33.33%(5/15),HPV-DNA阳性者ILK蛋白阳性表达率显著高于HPV-DNA阴性者(P<0.05)。HPV-DNA阳性者FAK蛋白阳性表达率为76.0%(57/75),HPV-DNA阴性者FAK蛋白阳性表达率为20.0%(3/15),HPV-DNA阳性者FAK蛋白阳性表达率显著高于HPV-DNA阴性者(P<0.05)。结论 CSCC组织中ILK和FAK蛋白表达上调，ILK和FAK蛋白表达与CSCC病变程度、HPV感染有相关性，ILK和FAK可能参与了CSCC的发生和发展。更多还原

【Abstract】 Objective To investigate the expression of integrin linked kinase(ILK) and focal adhesion kinase(FAK) in cervical squamous cell carcinoma(CSCC) tissues and their relationship with the clinicopathological features of CSCC and human papilloma virus(HPV) infection.Methods The specimens of 30 cases of normal cervical tissues, 30 cases of cervical intraepithelial neoplasia(CIN) tissues and 60 cases of CSCC tissues preserved in Handan First Hospital from October 2015 to August 2019 were selected.The expressions of ILK and FAK mRNA in normal cervical tissues, CIN tissues and CSCC tissues were detected by real-time fluorescence quantitative polymerase chain reaction, and the expressions of ILK and FAK protein in normal cervical tissues, CIN tissues and CSCC tissues were detected by immunohistochemistry.The relationship between the expression of ILK,FAK protein and clinicopathological features and HPV infection in CSCC patients was analyzed.Results The relative expression levels of ILK and FAK mRNA in CSCC tissues were 12.37±1.26 and 17.32±3.21 respectively, those in CIN tissues were 8.39±3.20 and 13.35±2.34 respectively, and those in normal cervical tissues were 3.67±2.40 and 5.47±2.10 respectively.The relative expression levels of ILK and FAK mRNA in CIN and CSCC tissues were significantly higher than those in normal cervical tissues(P<0.05),and the relative expression levels of ILK and FAK mRNA in CSCC tissues were significantly higher than those in CIN tissues(P<0.05).The positive expression rates of ILK and FAK proteins in normal cervical tissues were 6.67%(2/30) and 3.33%(1/30) respectively, and those in CIN tissues were 63.33%(19/30) and 56.67%(17/30) respectively, and those in CSCC tissues were 85.00%(51/60) and 71.67%(43/60) respectively.The positive expression rates of ILK and FAK protein in CSCC tissues were significantly higher than those in CIN and normal cervical tissues(P<0.05),and the positive expression rates of ILK and FAK protein in CIN tissues were significantly higher than those in normal cervical tissues(P<0.05).The expressions of ILK and FAK protein were related to the clinical stage and lymph node metastasis of CSCC(P<0.05),while they were not related to the age of CSCC patients(P>0.05).In CSCC and CIN tissues, HPV-DNA was positive in 75 cases, and HPV-DNA was negative in 15 cases.The positive expression rate of ILK protein in HPV-DNA positive patients was 86.67%(65/75),and it in HPV-DNA negative patients was 33.33%(5/15).The positive expression rate of ILK protein in HPV-DNA positive patients was significantly higher than that in HPV-DNA negative patients(P<0.05).The positive expression rate of FAK protein in HPV-DNA positive patients was 76.0%(57/75),and it in HPV-DNA negative patients was 20.0%(3/15).The positive expression rate of FAK protein in HPV-DNA positive patients was significantly higher than that in HPV-DNA negative patients(P<0.05).Conclusion The expressions of ILK and FAK proteins were up-regulated in CSCC tissues, and they were correlated with the degree of CSCC lesions and HPV infection.ILK and FAK may be involved in the occurrence and development of CSCC.更多还原