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标本溶血对电化学发光法检测生长激素结果的影响

Effects of Hemolysis of Specimens on the Results of Electrochemiluminescence Assay for Growth Hormone

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【作者】 程双喜柯丹丹江转南杨丽娟李玉凤

【Author】 CHENG Shuangxi;KE Dandan;JIANG Zhuannan;YANG Lijuan;LI Yufeng;Child Health Department,Dongguan Maternal and Child Health Hospital;Lanwei Medical Laboratory;Community Health Service Center,Songshan Lake High-tech Industrial Development Zone;

【机构】 东莞市妇幼保健院儿童保健科东莞市兰卫医学检验实验室东莞市松山湖高新技术产业开发区社区卫生服务中心

【摘要】 目的 探讨标本溶血对电化学发光法检测生长激素结果的影响。方法 收集149例生长激素检测值>5 ng/mL的正常血清样本,用离心机处理后人为造成样本重度溶血,并于溶血后30 min、8 h用电化学发光法测定其结果。结果 99例样本在重度溶血后30 min的检测值较其正常值低(0.22±0.60)ng/mL,差异有统计学意义(t=4.80,P<0.05);50例样本在重度溶血后8 h的检测值较其正常值低(0.19±0.27)ng/mL,差异有统计学意义(t=4.80,P<0.05)。结论 标本溶血会导致电化学发光法检测生长激素值稍低于正常水平。

【Abstract】 Objective To investigate the effect of specimen hemolysis on the results of electrochemiluminescence assay for growth hormone. Methods A total of 149 normal serum samples with growth hormone detection value greater than 5 ng/mL were collected. After centrifugation, the samples were artificially severely hemolyzed, and the results were determined by electrochemiluminescence 30 min and 8 h after hemolysis. Results The detection value of 99samples at 30 min after severe hemolysis was lower than the normal value(0.22±0.60)ng/mL, and the difference was statistically significant(t=4.80, P<0.05). The detection value of 50 samples at 8 h after severe hemolysis was lower than the normal value(0.19 ±0.27)ng/mL, and the difference was statistically significant(t =4.80, P <0.05).Conclusion The hemolysis of the specimen may cause the growth hormone value detected by electrochemiluminescence method to be slightly lower than the normal level.

【基金】 东莞市科技发展项目(202050715007852)
  • 【分类号】R446.1
  • 【下载频次】42
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