【摘要】 背景:尽管大多数骨折能够治愈,但仍有5%-10%最后发展为骨不连,临床试验证实高压氧可以通过血液循环显著提高体内动脉血氧分压,促使新陈代谢,加速骨愈合。目的:探讨高压氧干预不同时间对家兔股骨骨折愈合的影响和相关机制。方法:12只新西兰大白兔建立股骨骨折模型,随机分成高压氧治疗1个月组、高压氧治疗2个月组、常氧治疗1个月组、常氧治疗2个月组,每组3只。高压氧治疗组于术后第1天开始将所有动物置入高压氧动物仓中行高压氧治疗1,2个月,采用酶联免疫吸附法检测血清骨钙素、Ⅰ型胶原C端肽水平,苏木精-伊红染色观察骨折愈合情况。结果与结论:(1)治疗1个月时,高压氧组血清骨钙素、Ⅰ型胶原C端肽水平与常氧组无明显差异(P> 0.05);治疗2个月时,与常氧组比较,高压氧组血清Ⅰ型胶原C端肽水平明显减少(P <0.001)、骨钙素水平明显上升(P <0.01);(2)治疗1个月时,高压氧组与常氧组病理损伤程度无差异,治疗2个月时,高压氧组病理损伤程度较常氧组明显减轻;(3)结果表明,高压氧治疗2个月可有效提高骨形成指标骨钙素水平,提示可能促进成骨细胞形成进而对骨折愈合有积极作用。更多还原

【Abstract】 BACKGROUND:Most fra ctures can be healed,5%-10% of which will develop into nonunion.Clinical trials have confirmed that hyperbaric oxygen can accelerate fracture healing by significantly increasing the partial pressure of arte rial oxygen via the blood circulation and promoting metabolism.OBJECTIVE:To explore the effect and mechanism of hyperbaric oxygen intervention for different time on the healing of femoral fra ctures in rabbits.METHODS:Twelve New Zealand white rabbits were selected to make femoral fracture models and randomly divided into a hyperbaric oxygen treatment group for 1 month,a hyperbaric oxygen treatment group for 2 months, a normoxia treatment group for 1 month,and a normoxia treatment group for 2 months(3 rabbits per group).In the hyperbaric oxygen treatment groups,all animals were placed in a hyperbaric animal cabin for hyperbaric oxygen treatment on the1 st day after modeling.After 1 and 2 months of treatment enzyme-linked immunosorbent assay was used to detect the levels of serum osteocalcin and type Ⅰcollagen C-terminal peptide and hematoxylin-eosin staining was used to observe fracture healing.RESULTS AND CONCLUSION:After 1 month of treatment,expression levels of osteocalcin and type Ⅰ collagen C-terminal peptide in serum showed no significant difference between the hyperbaric oxygen treatment groups and normoxia treatment groups(P> 0.05).After 2 months of treatment,the serum level of type Ⅰ collagen C-terminal peptide was significantly decreased(P <0.001) and the serum level of osteocalcin was significantly increased(P <0.01) in the hyperbaric oxygen treatment group than the normoxia treatment group.After 1 month of treatment,there was no difference in the severity of pathological injury between the hyperbaric oxygen treatment group and normoxia group.Whereas,after 2 months of treatment,pathological injuries were significantly reduced in the hyperbaric oxygen treatm ent group compared with the norm oxia treatment group.To conclude,hyperbaric oxygen treatment for 2 months can effectively increase the expression of osteocalcin,suggesting that it may promote the formation of osteoblasts and accelerate fracture healing.更多还原