【摘要】 背景:神经导管桥接神经间隙替代自体神经移植修复周围神经损伤的报道很多,鼻黏膜来源外胚层间充质干细胞是移植治疗周围神经损伤的重要种子细胞。目的:研究鼻黏膜来源外胚层间充质干细胞与植物源神经导管的生物相容性及其在导管表面的自发分化生长情况。方法:取自然界内径为0.2 cm的植物管状茎秆,用物理和化学方法去除茎秆的肉质,保留其纤维性管状外形,用层粘连蛋白以京尼平交联修饰植物导管表面;体外分离和培养鼻黏膜来源外胚层间充质干细胞,并分为3组,分别种植于空白培养板、未修饰导管及修饰导管表面,于1,3,5,7,14 d以MTT法检测细胞活性,体外评估二者的生物相容性;细胞种植并培养2周后以细胞蛋白免疫印迹及免疫荧光实验观察该导管对鼻黏膜来源外胚层间充质干细胞自发分化的影响。结果与结论:(1)鼻黏膜来源外胚层间充质干细胞在植物导管纤维表面生长良好且成定向排列,植物导管与鼻黏膜来源外胚层间充质干细胞具有良好的生物相容性;(2)经层粘连蛋白修饰的导管表面的鼻黏膜来源外胚层间充质干细胞表达MBP和S100,且均高于空白组及未修饰导管组;(3)结果表明,层粘连蛋白修饰的植物源神经导管可促进鼻黏膜来源外胚层间充质干细胞向许旺细胞分化,可为天然植物源神经导管负载种子细胞移植修复周围神经缺损提供新的理论依据。更多还原

【Abstract】 BACKGROUND: There are many reports on the repair of peripheral nerve injury by nerve conduit bridging nerve gap instead of autogenous nerve graft. The nasal ectomesenchymal stem cell is an important seed cell for the transplantation of peripheral nerve injury.OBJECTIVE: To investigate the biocompatibility of nasal ectomesenchymal stem cells and plant-derived neural conduits and the spontaneous differentiation and growth of nasal ectomesenchymal stem cells on the surface of the conduit.METHODS: Tube culms with an inner diameter of 0.2 cm were selected in nature. After physical and chemical removal of pulp, their fibrous tubular shape was retained. The extracellular matrix protein laminin was modified by genipin. Nasal ectomesenchymal stem cells were isolated and cultured in vitro, and divided into three groups: the blank group(blank culture plate), the unmodified group(unmodified conduit) and the modified group(modified conduit). MTT assay was performed at 1, 3, 5, 7, and 14 days to evaluate cell viability. Biocompatibility was assessed in vitro. The effect of the conduit on the spontaneous differentiation of nasal mucosa-derived ectodermal mesenchymal stem cell was observed by immunoblotting and immunofluorescence two weeks after cell implantation and culture. RESULTS AND CONCLUSION:(1) Nasal ectomesenchymal stem cells grow well and were oriented on the surface of plant duct fibers, and plant conduits were biocompatible with nasal ectomesenchymal stem cells.(2) The expression levels of MBP and S100 in nasal ectomesenchymal stem cells implanted on the surface of the catheter were higher than those in the blank and unmodified groups.(3) It is concluded that the differentiation of nasal ectomesenchymal stem cells into Schwann cells can be promoted by laminin-modified plant-derived nerve conduits, which may provide a new theoretical basis for the transplantation and repair of peripheral nerve cells loaded with natural plant-derived nerve conduits.更多还原