【摘要】 目的 观察细胞间黏附分子-1（ICAM-1）在牙周炎、动脉粥样硬化及牙周炎伴动脉粥样硬化大鼠血清和牙周组织中的表达情况，探讨牙周炎与动脉粥样硬化之间的关系。方法 选取40只Wistar雄性大鼠，随机分为正常组、牙周炎组、动脉粥样硬化组和牙周炎伴动脉粥样硬化组，每组10只。正常组大鼠只给予普通饲料喂食；牙周炎组大鼠在颊腭侧龈沟下注射内毒素混悬液造模，每周1次，共3次，实验过程中给予普通饲料喂养；动脉粥样硬化组大鼠实验第1周给予普通饲料喂养，同期一次性腹腔注射维生素D₃注射液7×10⁵ IU/kg, 1周后逐步添加高脂饲料直至第2周末完全替代普通饲料；牙周炎伴动脉粥样硬化组同时给予牙周炎组和动脉粥样硬化组相同的干预。比较各组大鼠实验前及喂养12周末的体重、喂养13周末牙龈出血指数和探诊深度；喂养13周末处死各组大鼠，提取血液和上颌骨、颈总动脉组织，HE染色观察各组大鼠牙周组织和颈总动脉病理形态，酶联免疫吸附法检测血清ICAM-1水平，免疫组化法检测牙周组织中ICAM-1表达情况。结果 喂养12周末，4组大鼠体重均明显高于实验前，但牙周炎组、动脉粥样硬化组和牙周炎伴动脉粥样硬化组均明显低于正常组（P均<0.05）,且牙周炎伴动脉粥样硬化组均明显低于牙周炎组和动脉粥样硬化组（P均<0.05）。喂养13周末，牙周炎组和牙周炎伴动脉粥样硬化组大鼠牙龈出血指数和探诊深度均明显高于正常组和动脉粥样硬化组（P均<0.05）,牙周炎伴动脉粥样硬化组均明显高于牙周炎组（P均<0.05）。HE染色见牙周炎伴动脉粥样硬化组牙龈上皮内炎症重于牙周炎组，血管炎症重于动脉粥样硬化组。牙周炎组、动脉粥样硬化组和牙周炎伴动脉粥样硬化组大鼠血清ICAM-1水平和牙周组织中ICAM-1阳性表达平均光密度值均明显高于正常组（P均<0.05）,且牙周炎伴动脉粥样硬化组均明显高于牙周炎组和动脉粥样硬化组（P均<0.05）。结论 牙周炎和动脉粥样硬化大鼠血清和牙周组织中ICAM-1表达均较高，即两种疾病均存在明显炎症反应，二者之间可能有互相影响、互相促进的关系。更多还原

【Abstract】 Objective It is to observe the expression of intercellular adhesion molecule-1(ICAM-1) in the serum and periodontal tissues of rats with periodontitis, with atherosclerosis and with periodontitis and atherosclerosis, to investigate the relationship between periodontitis and atherosclerosis. Methods Forty male Wistar rats were selected and randomly divided into normal group, periodontitis group, atherosclerosis group and periodontitis with atherosclerosis group, 10 rats in each group. The rats in the normal group were fed with normal fodder; the rats in the periodontitis group were injected with endotoxin suspension under the gingival sulcus on the buccal and palatal sides, once a week, totally for 3 times, and fed with normal fodder during the experiment. The rats in the atherosclerosis group were fed with normal fodder in the first week of the experiment, and was injected intraperitoneally with vitamin D3 injection 7×10~5 IU/kg at the same time, and high-fat fodder was added gradually after 1 week until the end of the second week. The rats in the periodontitis with atherosclerosis group were given the same intervention as the periodontitis group and atherosclerosis group. The body weight of the rats in each group before the experiment and at the end of 12 weeks’ feeding, the gingival bleeding index and probing depth at the end of 13 weeks’ feeding were compared. The rats in each group were executed at the end of 13 weeks of feeding, their blood and maxillary and common carotid artery tissues were extracted, and the pathological morphology of periodontal tissues and common carotid artery in each group was observed by HE staining, the level of serum ICAM-1 was detected by enzyme-linked immunosorbent assay, and the expression of ICAM-1 expression in periodontal tissues was detected by immunohistochemistry. Results At the end of 12 weeks’ feeding, the body weight of the rats in all four groups was significantly higher than that before the experiment, but the periodontitis group, the atherosclerosis group and the periodontitis with atherosclerosis group were significantly lower than the normal group(all P<0.05), and the periodontitis with atherosclerosis group was significantly lower than the periodontitis group and the atherosclerosis group(all P<0.05). At the end of 13 weeks of feeding, the gingival bleeding index and probing depth of rats in the periodontitis group and periodontitis with atherosclerosis group were significantly higher than those in the normal group and atherosclerosis group(all P<0.05), and those in the periodontitis with atherosclerosis group were significantly higher than those in the periodontitis group(all P<0.05). HE staining showed that the gingival intraepithelial inflammation was more serious in the periodontitis with atherosclerosis group than that in the periodontitis group, and the vascular inflammation was more serious than that in the atherosclerosis group. The serum ICAM-1 levels and the mean optical density values of positive ICAM-1 expression in periodontal tissues were significantly higher in the periodontitis group, atherosclerosis group and periodontitis with atherosclerosis group than those in the normal group(all P<0.05), and the periodontitis with atherosclerosis group was significantly higher than the periodontitis group and atherosclerosis group(all P<0.05). Conclusion ICAM-1 expression was higher in both serum and periodontal tissues of rats with periodontitis and atherosclerosis, i.e., there was a significant inflammatory response in both diseases, and there may have a relationship of mutual influence and mutual promotion.更多还原