【摘要】 为了改良和利用盐碱地，以秋茄(Kandelia obovata)和木榄(Bruguiera gymnorhiza)为原材料，对耐盐内生细菌进行分离并鉴定。结果表明：从2种植物的根、茎、叶中初步分离出耐盐的内生细菌96株，均为革兰氏阳性菌；提取各菌株的DNA并扩增其16S rDNA序列，以HinfⅠ、HaeⅢ、MspⅠ和TaqⅠ4种酶进行酶切，并统计遗传型，选取不同组合类型的代表菌株进行16S rDNA序列测定，通过BLAST比对分析并利用MEGA软件建立发育树等分子鉴定手段初步推断，所分离的内生细菌分别为越南芽孢杆菌(Bacillus vietnamensis)、阿耶波多氏芽孢杆菌(Bacillus aryabhattai)、芽孢杆菌属(Bacillus sp.)、白翎芽孢杆菌(Bacillus baekryungensis)和巴氏葡萄球菌(Staphylococcus pasteuri);对这5种菌的脱氨酶活性进行测定，越南芽孢杆菌的值最高。更多还原

【Abstract】 In order to ameliorate and utilize saline alkali soil, endophytes of kandelia obovata and Bruguiera gymnorhiza were isolated and identificated by combining physiological analysis and molecular identification.The results showed that 96 strains of salt-tolerant endophytic bacteria were preliminarily isolated from the roots and leaves of two plants, all of which were Gram-positive bacteria.The DNA of each strain was extracted, 16 S rDNA sequence by PCR,four enzymes were used for enzyme digestion, and the genetic types were counted.16 S rDNA sequence determination of representative strains of different combination types was carried out, blast analysis, phylogenetic tree using establishing by MEGA software, the isolated endophytic bacteria were Bacillus vietnamensis,Bacillus aryabhattai,Bacillus baekryungensis,Bacillus ceurus and Staphylococcus pasteuri.The ACC deaminase activity of these five bacteria was determined, and the value of Bacillus vietnamensis was the highest.更多还原