【摘要】 目的 用高效液相色谱二极管阵列器法（high performance liquid chromatography photodiode array, PLC-PDA）建立小血藤指纹图谱和同时测定小血藤中3种成分含量的分析方法，评价24批不同产地小血藤的质量。方法 用Shim-pack Scepter C₁₈液相色谱柱（250 mm×4.6 mm, 5μm）;以乙腈（A）-1 mL·L^-1磷酸水溶液（B）为流动相，梯度洗脱；检测波长为300 nm。指纹图谱数据导入SPSS 26.0与SIMCA 14.1统计软件进行聚类分析、主成分分析和正交偏最小二乘法判别分析，以变量投影重要性值大于1为标准，筛选影响小血藤质量的差异标志物，并以高效液相色谱法（high performance liquid chromatography, HPLC）测定其中芦丁、紫云英苷、槲皮素的含量。结果 24批小血藤指纹图谱发现共有峰22个，通过与对照品比对指认3个峰；陕南样品的相似度均高于0.92,其余样品的相似度均低于0.92;24批小血藤可聚为2类，S1～S16、S21、S22聚为Ⅰ类，S17～S19、S23、S24聚为Ⅱ类；前5种主成分的方差贡献率分别为36.16%、19.03%、17.47%、10.93%、8.30%,累积方差贡献率为91.89%;芦丁、紫云英苷、17号峰、6号峰、13号峰5种成分为影响小血藤质量的差异标志物；芦丁、紫云英苷、槲皮素的含量分别为0.272 8～0.476 4、0.058 3～0.155 8、0.042 1～0.057 4 mg·g^-1。结论 建立的HPLC指纹图谱和含量测定方法简便、稳定、可靠，可用于小血藤的质量控制。更多还原

【Abstract】 Objective To establish high performance liquid chromatography photodiode array（HPLC-PDA） fingerprints of 24 batches of Schisandra propinqua var. sinensis,determine the contents of 3 components, and therefore to evaluate the quality of 24 batches of the medicinal materials from different producing areas.Methods A Shim-pack Scepter C₁₈ column（250 mm×4.6 mm, 5 μm） was used with a gradient of acetonitrile（A）-1 mL·L^-1 phosphoric acid（B） as mobile phase. Fingerprints data were imported into SPSS 26.0 and SIMCA-P 14.1 software for cluster analysis, principle component analysis and orthogonal partial least squares-discrimination analysis. According to the standard of the variable importance in the project above 1,the differential markers affecting the quality difference of Schisandra propinqua var. sinensis were screened. At the same time, the contents of the 3 components including rutin, astragaloside, quercetin were determined.Results 22 common peaks were found in the fingerprints of 24 batches of Schisandra propinqua var. sinensis,and 3 peaks were identified by standard compounds. The similarity of samples from southern Shaanxi was more than 0.92,and the similarity of other samples was less than 0.92. These 24 batches of samples could be classified into 2 categories（S1-S16,S21 and S22 into a class, and the others clustered into another one）. By principal component analysis, the contribution rates of 5 principle components were 36.16%,19.03%,17.47%,10.93% and 8.30%,respectively. The accumulative contribution rate was 91.89%. The 5 constituents, such as rutin, astragaloside, peak 17,peak 6,peak 13 were screened as differential markers representing the major differences of Schisandra propinqua var. sinensis. The contents of rutin, astragaloside and quercetin were 0.272 8-0.476 4,0.058 3-0.155 8 and 0.042 1-0.057 4 mg·g^-1 respectively.Conclusion The HPLC fingerprint and content determination method are simple,stable and reliable,and can be used for the quality control of Schisandra propinqua var. sinensis.更多还原