【摘要】 绿色合成的纳米硒颗粒越来越受到人们的关注.使用干酪乳杆菌和嗜酸乳杆菌在厌氧条件下生物合成硒纳米颗粒(Selenium nanoparticles, SeNPs).利用溶菌酶和超声波对细菌细胞裂解，采用有机-水萃取体系提取胞内SeNPs并进行表征.分析表明，SeNPs在4℃下比较稳定，颗粒均匀，分散性好，带负电荷，呈非晶态结构.颗粒表面含有蛋白质、脂质和糖类等物质.此外，SeNPs对荧光假单胞菌(Pseudomonas fluorescens)模式菌的最小抑菌浓度(Minimum inhibitory concentration, MIC)分别为0.1 mg/mL和0.2 mg/mL,对荧光假单胞菌野生菌的MIC都为0.2 mg/mL.硒纳米颗粒对供试菌的生长均有抑制作用，随着SeNPs浓度的增大，生物膜抑制率达到43.52±1.23%和55.83±1.14%.硒纳米颗粒有望成为一种安全环保、有效的抗菌剂，应用于冷藏食品中.更多还原

【Abstract】 Nowadays, more and more attention has been paid to the green synthesis of selenium nanoparticles(SeNPs).In this study, SeNPs were biosynthesized by Lactobacillus casei and Lactobacillus acidophilus under anaerobic conditions.Using of lysozyme and ultrasonication for bacterial cell lysis, intracellular SeNPs were extracted by an organic-aqueous extraction system and then characterized by UV-visible, X-ray diffraction, and Fourier transform infrared spectroscopy analyses.The analysis of SeNPs were relatively stable at 4 ℃,with uniform particles, good dispersibility, amorphous structure.As observed the particles have a negative charge.The surface of SeNPs contained proteins, lipids and sugars.In addition, the minimum inhibitory concentrations(MIC) of SeNPs against Pseudomonas fluorescens model bacteria were 0.1 mg/mL and 0.2 mg/mL,respectively, and 0.2 mg/mL for Pseudomonas fluorescens wild bacteria. Selenium nanoparticles inhibited the growth of the tested bacteria.With the increase of SeNPs concentration, the inhibition rate of biofilm reached 43.52±1.23% and 55.83±1.14%.SeNPs will be expected to be a safe, environmentally friendly and effective antibacterial agent, which can be used in refrigerated food.更多还原