【摘要】 为提高昆虫病原真菌莫勒菌产孢量，通过正交试验设计L₉（3⁴），对莫勒菌培养基成分进行筛选和优化。结果表明，有助于莫勒菌产孢的碳源、氮源、金属离子和维生素分别为7 g·L^-1可溶性淀粉、10 g·L^-1酵母浸粉、4×10^-4mol·L^-1Fe²⁺和50 mg·L^-1V_C。莫勒菌在优化培养基上培养2周后的产孢量可达1.2×10⁸孢子·cm^-2，比优化前提高60倍。更多还原

【Abstract】 To improve sporulation,the composition of the growth medium for the entomopathogenic fungus Moelleriella raciborskii was optimized based on the orthogonal layout method including three levels of four variables.Specifically,7 g·L^-1of soluble starch,10 g·L^-1of yeast powder,4×10^-4mol·L^-1of ferrous ion,and 50 mg·L^-1of ascorbic acid were the best sources of carbon,nitrogen,metal ions,and vitamins,respectively,for M.raciborskii sporulation.After 2 weeks of culture in this optimized medium,the spore production of M.raciborskii peaked,reaching1.2×10⁸conidia·cm^-2.Conidium production in the optimized medium was approximately 60-fold higher than that in the nonoptimized basal medium.更多还原