【摘要】 硒（Ⅳ）与DAN（2,3-二氨基萘）生成具有荧光特性的4,5-苯并苤硒脑，可用分子荧光光谱法测定，优化测定的实验条件，建立了快速准确测定大米中痕量硒的方法。结果表明，其最佳测定条件为：pH=2.0,反应温度为80℃,1.0×10^-5mol/L十六烷基溴化吡啶用作表面活性剂，环己烷萃取条件下，增敏效果明显，在0～0.067μg/mL浓度范围内线性关系良好，方法检出限为6.5×10^-4μg/mL,并且将方法应用于三个不同地区的大米中硒含量的测定，相对标准偏差为1.9%～2.8%,加标回收率在87.8%～107%,表明方法准确可靠。更多还原

【Abstract】 Trace Se can be determined by molecular fluorescence spectrophotometry when selenium（Ⅳ） with DAN（2,3-diaminonaphthalene） was carried out to produce 4,5-benzoselenol with strong fluorescence characteristics.The conditions for the determination of trace Se were optimized, and the fast determination of trace Se in selenium-enriched rice were established.The results showed that the optimal test conditions were as the follow: pH=2.0,80 ℃,1.0×10^-5 mol/L CPB in cyclohexane, the sensitization effect was obvious.Under the optimum conditions, a good linearity was observed in the range of 0—0.067 μg/mL with the detection limit is 6.5×10^-4 μg/mL.The proposed method was applied to the determination of the trace selenium in selenium-enriched rice from three different provinces, the RSD of the determination results were 1.9%—2.8%.The recoveries of samples were 87.8%—107%,the result showed the method is reliable and sensitive.更多还原