【摘要】 背景:免疫功能失调在炎症性肠病(IBD)的发病机制中起着关键作用。羌活醇是中药羌活的主要活性成分，具有抗炎的作用。目的:探究羌活醇对葡聚糖硫酸钠(DSS)诱导的小鼠结肠炎的作用。方法:将C57BL/6小鼠随机分为正常组、阴性对照组、模型组、羌活醇组。模型组、羌活醇组小鼠自由饮用2%DSS溶液诱导结肠炎模型。阴性对照组、羌活醇组小鼠给予腹腔注射羌活醇溶液40 mg/kg,正常组、模型组小鼠给予腹腔注射等体积0.9%NaCl溶液。10 d后处死小鼠，行疾病活动指数(DAI)评分、结肠长度和组织病理学评分，ELISA法检测结肠组织中TNF-α、IL-1β、IL-6、IL-17A水平，定量PCR法检测结肠和脾脏组织中IL-17A、RORγt mRNA表达。结果:与正常组相比，模型组DAI评分、组织病理学评分、结肠组织中TNF-α、IL-1β、IL-6、IL-17A水平、结肠和脾脏组织中IL-17A、RORγt mRNA表达均显著增高(P<0.01),结肠长度明显缩短(P<0.01)。与模型组相比，羌活醇能明显降低小鼠DAI评分、组织病理学评分(P<0.01),下调TNF-α、IL-1β、IL-6、IL-17A水平(P<0.01),抑制IL-17A、RORγt mRNA表达(P<0.01),明显恢复结肠长度(P<0.01)。结论:羌活醇对小鼠结肠炎有保护作用，其机制可能与减少促炎细胞因子的产生、抑制Th17细胞的功能和分化相关。更多还原

【Abstract】 Background: Immunological dysfunction plays a key role in the pathogenesis of inflammatory bowel disease(IBD). Notopterol is the main ingredient of the traditional Chinese herb medicine Notopterygium incisum Ting ex H.T. Chang and has anti-inflammatory effect. Aims: To explore the effect of notopterol on dextran sulfate sodium(DSS)-induced colitis in mice. Methods: C57BL/6 mice were randomly divided into normal group, negative control group, model group and notopterol group. Mice in model group and notopterol group were treated with 2% DSS to induce colitis. Mice in negative control group and notopterol group were injected intraperitoneally with notopterol 40 mg/kg, and mice in normal group and model group were injected intraperitoneally with 0.9% NaCl solution. Mice were sacrificed 10 days later, and disease activity index(DAI) score, colon length and histopathological score were determined. The levels of TNF-α, IL-1β, IL-6, IL-17A were assessed by ELISA. The mRNA expressions of IL-17A, RORγt were detected by quantitative PCR. Results: Compared with the normal group, DAI score, histopathological score, levels of TNF-α, IL-1β, IL-6, IL-17A, mRNA expressions of IL-17A and RORγt in model group were significantly increased(P<0.01), and colon length were significantly shortened(P<0.01). Compared with the model group, notopterol significantly reduced DAI score and histopathological score(P<0.01), downregulated levels of TNF-α, IL-1β, IL-6, IL-17A(P<0.01), inhibited the mRNA expressions of IL-17A, RORγt(P<0.01), and greatly recovered colon length(P<0.01). Conclusions: Notopterol has protective effects on DSS-induced colitis in mice. The mechanism may be related to reducing the secretion of pro-inflammatory cytokines and inhibiting the function and differentiation of Th17 cells.更多还原