【摘要】 为了探究S1Fa-like转录因子在玉米生长发育及响应非生物胁迫中的作用，对玉米S1Fa-like TFs进行了鉴定，分析了蛋白的理化性质、基因结构、染色体定位、进化关系、启动子顺式调控元件等，同时基于转录组的结果研究了组织表达以及干旱、高温等胁迫表达模式，并利用qRT-PCR技术对高温胁迫表达模式进行了检测。结果显示：在玉米中共鉴定到2个ZmS1Fas基因，其编码5个蛋白，均定位在细胞核内。对14种作物的S1Fa-like蛋白氨基酸序列进行多重比对，发现单子叶植物和双子叶植物的S1Fa-like蛋白具有高度保守性。ZmS1Fas启动子区域含有丰富的与激素响应和非生物胁迫响应相关的调控元件。ZmS1Fas在玉米组织均有表达，且多数组织中ZmS1Fa2的表达量比ZmS1Fa1的表达量高。受到干旱胁迫时，ZmS1Fas表达量变化不大。自交系Oh43和杂交种Oh43×B73受高温胁迫，ZmS1Fas均显著上调表达；自交系PH207受低温胁迫，ZmS1Fas均显著下调表达。qRT-PCR检测结果表明：ZmS1Fa1和ZmS1Fa2在B73中均受热胁迫轻微下调表达，而在Oh43中受热胁迫显著上调表达。不同玉米材料中ZmS1Fas响应温度胁迫模式不同，为耐高温玉米品系的遗传改良提供潜在的基因资源，为进一步解析ZmS1Fas在响应非生物胁迫应答中的生物学功能奠定基础。更多还原

【Abstract】 In order to investigate the role of S1Fa-like transcription factors in maize growth, development and abiotic stresses responses, we identified the S1Fa-like TFs from the Zea mays genome, and analyzed physicochemical properties of protein, genes structure,chromosomal localization, phylogenetic tree andcis-elements systematically. The gene tissue expression and the expression patterns under stresses such as drought and high temperature were also studied based on the results of transcriptome. Meanwhile, the expression patterns of high temperature stresses were examined by qRT-PCR. The results showed that a total of two ZmS1Fas, encoding five proteins, were identified in maize, all localized in the nucleus. Sequence alignment of S1Fa-like proteins from 14 crops revealed that S1Fa-like TFs were highly conserved in monocots and dicots. The promoter regions of ZmS1Fas were rich in cis-elements associated with hormone response and abiotic stresses. ZmS1Fas were expressed in all maize tissues, and the expression level of ZmS1Fa2 was higher than that of ZmS1Fa1 in most tissues. When subjected to drought stress, the expressions of ZmS1Fas were not significantly different from that in the normal growth material at the transcript level. However, the expressions of ZmS1Fas were significantly up-regulated in both Oh43 and Oh43×B73 under high temperature stress, while the expressions of ZmS1Fas were significantly down-regulated in PH207 under low temperature stress. qRT-PCR analysis showed that both ZmS1Fa1 and ZmS1Fa2 were slightly down-regulated by heat stress in B73, while they were significantly up-regulated by heat stress in Oh43. The different patterns of ZmS1Fas response to temperature stress in different maize materials provide potential genetic resources for the genetic improvement of heat-tolerant maize lines and lay the foundation for further elucidation of the biological functions of ZmS1Fas in response to abiotic stress responses.更多还原