【摘要】 对2018-2019年4个批次送检的病死雏鹅进行病原菌分离鉴定，并对其相关耐药性和毒力基因进行检测。采用病死鹅肝脏组织进行病原菌的分离鉴定、药敏试验及耐药和毒力基因检测。结果表明：4株菌均为鲍曼不动杆菌。4株均扩增出耐β-内酰胺酶基因blatem-1和耐磺胺类基因sul1。1株扩增出含有耐头孢菌素类基因blaCMY-2。4株均含有编码铁摄取系统基因basJ、外膜蛋白基因OmpA。3株扩增出磷脂酶D基因plcD。4株均未扩增出生物被膜形成蛋白基因。更多还原

【Abstract】 Pathogenic bacteria were isolated and identified from four batches of dead goslings sent for examination from 2018 to 2019, their related drug resistance and virulence genes were detected. The liver tissue of dead geese was used for isolation and identification of pathogens, drug sensitivity test,drug resistance and virulence gene detection. The results showed that the four strains were all Acinetobacter baumannii. The β-lactamase resistant gene blatem-1 and sulfamine-resistant gene sul1 were amplified from four strains. One cephalosporin-resistant gene blaCMY-2 was amplified. All the four strains contained basJ gene encoding iron uptake system and outer membrane protein gene OmpA.Three of them amplified plcD of phospholipase D gene. Biofilm-forming protein gene was not amplified in all the 4 strains.更多还原