【摘要】 目的：探讨微小RNA-500 (microRNA-500,miR-500)在肾细胞癌中表达及其生物学功能。方法：收集2020年1月至2021年2月在我院泌尿外科行手术治疗且经病理组织学确诊的36例肾细胞癌患者的肾癌组织及癌旁正常组织，采用荧光定量聚合酶链反应（qRT-PCR）技术检测癌组织与癌旁正常组织中miR-500表达水平。将miR-500在肾癌细胞系ACHN和KETR3细胞中过表达，采用qRT-PCR验证转染效率。采用MTS试验、划痕试验和流式细胞术检测过表达miR-500对肾癌细胞系ACHN和KETR3细胞增殖、迁移和凋亡的影响。结果：miR-500在肾癌组织的中的表达量低于癌旁正常组织（P<0.05）;qRT-PCR结果显示转染ACHN和KETR3细胞后，转染组miR-500的表达高于对应的对照组（P<0.01）；过表达miR-500可抑制ACHN和KETR3细胞增殖及迁移，同时能诱导肾癌细胞凋亡增加（P<0.05）。结论：miR-500可能作为肾细胞癌发生、发展的抑制因子，有望成为肾肿瘤生物治疗的靶点。更多还原

【Abstract】 Objective: To investigate the expression and biological function of microRNA-500(miR-500) in renal cell carcinoma. Methods:Renal cell carcinoma tissues and adjacent normal tissues of 36 patients with renal cell carcinoma from Jan 2020 to Feb 2021 who underwent surgery in our hospital and diagnosed by histopathology were collected. The expression of miR-500 in renal cell carcinoma tissues and adjacent normal tissues were detected by q RT-PCR. miR-500 was overexpressed in ACHN and KETR3 cell lines,and qRT-PCR was used to verify the transfection efficiency. The effects of miR-500 overexpression on proliferation, migration and apoptosis of ACHN and KETR3 cells were detected by MTS test, scratch test and flow cytometry. Results:The expression of miR-500 in renal cell carcinoma tissues was lower than that in adjacent normal tissues(P<0.05). qRT-PCR results showed that after transfection of ACHN and KETR3 cells,the expression of miR-500 in the transfection group was higher than that in the control group(P<0.01). Overexpression of miR-500 could inhibit the proliferation and migration of ACHN and KETR3 cells,and induce apoptosis of renal carcinoma cells(P<0.05). Conclusion:MiR-500 may act as an inhibitory factor for the occurrence and development of renal cell carcinoma,and it is expected to become a target of biological therapy for renal cell carcinoma.更多还原