【摘要】 凝胶电泳与蛋白质印迹技术是生命医学研究领域的常用技术，但因此项技术步骤较多、操作复杂，学生在实验过程中出现的问题较多，结果不稳定，重复性差。为使学生能熟练且稳定地掌握该技术，对十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）及蛋白质印迹技术进行了长期的实践和探索，通过改进凝胶各成分配比方案，不断完善和优化实验条件，有效增强了凝胶配制的时效性，大大提高了凝胶电泳后续实验的稳定性和成功率。通过优化蛋白印迹过程中抗体的使用浓度，印记杂交结果目标蛋白质条带更加清晰可见，易分析，重复性好。更多还原

【Abstract】 Gel electrophoresis and protein imprinting technology are commonly used in the field of life medicine research, but they have many steps and complicated operations, and students have many problems in the experiment process, with unstable results and poor repeatability.In order to enable students to master this technology skillfully and stably, long-term practice and exploration have been carried out on sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE) and protein imprinting technology. By improving the formula of gel components,constantly improving and optimizing experimental conditions, the timeliness of gel preparation has been effectively enhanced, and the stability and success rate of subsequent gel electrophoresis experiments have been greatly improved. By optimizing the concentration of antibody used in the process of protein imprinting, the target protein bands in the result of imprinting hybridization are more clearly visible, easy to analyze, and have good repeatability.更多还原