【摘要】 目的:研究LAM/ITGα6β1/FAK信号通路在大鼠腮腺主导管结扎后再通诱导萎缩性腮腺组织再生过程中的表达及作用机制。方法:建立大鼠腮腺主导管结扎后再通诱导腺体萎缩后再生动物模型。HE观察腮腺组织形态学变化，免疫组化、Western Blot、RT-PCR检测LAM/ITGα6β1/FAK mRNA和蛋白的动态变化。结果:LAM、ITGα6β1表达量在再通后先减低，而后上调，到达峰值后减低。FAK在腮腺再生过程中逐渐升高。结论:LAM、ITGα6β1、FAK表达与导管结扎后再通诱导腮腺组织再生的过程关系密切。更多还原

【Abstract】 Objective: To investigate the mechanism of LAM/ITGα6β1/FAK signaling pathway in the regeneration of atrophic parotid gland induced by recanalization after ligation of main duct in rats. Methods: The animal model of gland atrophy and regeneration induced by main duct ligation and recanalization of parotid gland in rats was established. Histological changes of the parotid gland were observed using HE staining. The mRNA and protein expression changes of laminin, integrin α6 subunit, integrin β1 subunit and focal adhesion kinase were detected by immunohistochemistry, Western blot and RT-PCR respectively. Results: After recanalization of main duct, the expression levels of LAM and ITGα6β1 decreased first, then increased, and decreased after reaching the peak value. The expression level of FAK increased continuously during parotid regeneration. Conclusion: The expression of LAM, ITGα6β1 and FAK is closely related to the process of regeneration induced by duct recanalization.更多还原