【摘要】 观察龙血竭对脑缺血大鼠的神经功能评分、脑水肿、脑梗死面积、细胞形态和氧化应激指标的影响，探讨其脑保护作用。采用线栓法建立大鼠大脑中动脉栓塞（MCAO）模型，将大鼠随机分为六组，连续给药14天，记录大鼠神经功能缺损评分（m NSS）；取脑组织做2,3,5-三苯基氯化四氮唑（TTC）染色测定脑梗死体积；测定脑组织含水量（%）；用大鼠血清进行超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-P）、丙二醛（MDA）氧化应激指标检测。结果与假手术组（Sham）相比，模型组的脑组织含水量、脑梗死面积、神经功能评分都高，氧化应激的指标SOD、GSH-Px、CAT活力显著性下降（P<0.05）,MDA含量升高。与模型（Model）组相比，给药组均能降低神经行为学评分，减少脑梗死体积，有显著性差异（P<0.05），且龙血竭高/中（DBH/M）组优于脑心通胶囊（NXT）组；给药组能降低脑组织含水量，升高SOD、GSH-Px、CAT活力，降低MDA含量，DBH组有显著性差异（P<0.05）,NXT组无显著性差异（P>0.05）。得出龙血竭能降低脑组织含水量，降低脑梗死面积，改善细胞形态，减少氧化应激反应和改善神经损伤而产生脑保护作用。更多还原

【Abstract】 In this study, we observed the effects of Langxue Jie on neurological function score, cerebral edema, cerebral infarction area, cell morphology and oxidative stress indexes of cerebral ischemia rats, and explored its brain protection effect. The middle cerebral artery embolization(MCAO) model of rats was established by the method of thread embolization. The rats were randomly divided into six groups, and the neurological deficit score(mNSS) of rats was recorded for 14 consecutive days. TTC staining was used to determine the volume of cerebral infarction. The water content of brain tissue was determined(%). SOD, CAT, GSH-Px and MDA oxidative stress indexes were detected in serum of rats. Compared with Sham, the cerebral tissue water content, cerebral infarction area and neurological function score in model group were higher, and the activities of SOD, GSH-Px and CAT, indicators of oxidative stress, were decreased with significant differences(P<0.05), while MDA content was increased. Compared with Model group, neurobehavioral score and cerebral infarction volume in DBH/M group were decreased significantly(P<0.05), and DBH/M group was superior to NXT group. In the treatment group, the water content of brain tissue was decreased, SOD, GSH-Px and CAT activities were increased, and MDA content was decreased. There was significant difference in DBH group(P<0.05), but no significant difference in NXT group(P>0.05). It is concluded that Longxue Jie can reduce the water content of brain tissue, reduce the area of cerebral infarction, improve cell morphology, reduce oxidative stress response and therefore improve nerve injury, and produce brain protection.更多还原