【摘要】 采用同时蒸馏萃取和气相色谱质谱联用法对采集自7个地区的10株蛹虫草(Cordyceps militaris)的发酵菌丝体进行挥发性成分分析，共鉴定到65种挥发性成分，主要包括酸类、酯类、萘、烷烃类、醛类、蒽等物质，在挥发性成分中的平均含量分别为71.16%、10.57%、5.25%、5.13%、4.04%、1.23%。所有样本中均检出亚油酸、棕榈酸、萘、正己醛、马索亚内酯、α-蒎烯、亚油酸甲酯、2-正戊基呋喃8种成分，平均含量分别为37.41%、29.09%、5.25%、3.26%、2.65%、2.27%、1.53%、0.65%。蛹虫草菌丝体中还检测到石竹素、α-毕橙茄醇、α-柏木烯、马索亚内酯等药用挥发性成分。主成分分析和聚类分析显示，云南地区和辽宁地区蛹虫草菌丝体挥发性成分差异较大。相对气味活度值分析表明，蛹虫草菌丝体中醛类对其气味贡献最大，正己醛和2-正戊基呋喃等可能是蛹虫草关键性香气成分；不同地区蛹虫草菌株的菌丝体气味特征存在差异。更多还原

【Abstract】 Using simultaneous distillation extraction(SDE) and gas chromatography-mass spectrometry(GC-MS),10 mycelium samples of Cordyceps militaris collected in seven places in China were measured for their volatile components.A total of 65 volatile components were identified,and they mainly included acids,esters,naphthalenes,alkanes,aldehydes and anthracenes with an average content of 71.16 %,10.57 %,5.25 %,5.13 %,4.04 % and 1.23 %,respectively.Eight components,linoleic acid,palmitic acid,naphthalene,hexanal,massoia lactone,α-pinene,methyl linoleate and 2-pentylfuran,were detected in all samples,and their average contents were 37.41 %,29.09 %,5.25 %,3.26 %,2.65 %,2.27 %,1.53 % and 0.65 %,respectively.Some typical medicinal volatile substances,such as caryophyllin,a-cadinol,a-cedrene,massoia lactone,were also detected.As shown by principal component analysis(PCA) and clustering analysis(CA),there were large differences in volatile components of samples from Yunnan and Liaoning.Relative odor activity value(ROAV) analysis showed that aldehydes contributed the most to the odor of C.militaris mycelium,and other key aroma components included hexanal and 2-pentylfuran.There were differences in mycelial odor characteristics of C.militaris from different production regions.更多还原