【摘要】 目的 探讨牛磺酸(Tau)对人宫颈癌细胞侵袭转移的影响及分子机制。方法 采用20～160 mmol/L不同浓度Tau处理宫颈癌Hela细胞24～72 h, CCK8检测细胞增殖活性，Transwell小室检测细胞侵袭与迁移能力，Western blot检测蛋白表达。结果 终浓度为40～160 mmol/L的Tau对人宫颈癌细胞增殖和侵袭转移有不同程度的抑制作用(P<0.05),且呈时效和量效关系；同时Tau能增加上皮-间质转化(EMT)代表上皮特征的E-cadherin表达，下调代表间质特征的N-cadherin, Vimentin蛋白及基质金属蛋白酶(MMPs)表达；抑制AKT/GSK-3β通路及降低β-catenin蛋白含量。结论 Tau具有抑制人宫颈癌细胞侵袭转移与EMT的作用，其机制可能与AKT/GSK-3β/β-catenin信号通路有关。更多还原

【Abstract】 Objective To investigate the effect and mechanism of taurine(Tau) on invasion and metastasis of human cervical cancer cells.Methods Cervical cancer Hela cells were treated with Tau at different concentrations for 24～48 h, the proliferative activity of the cells was detected by CCK8;the ability of cell migration and invasion were detected by Transwell assay; Western blot was used to detect protein expression in the cells.Results The results showed that Tau(final concentration 40～160 mmol/L) can inhibit the proliferation, invasion and migration of cervical cancer cells to varying degrees(P<0.05),And showed a certain dose-effect relationship; Meanwhile, Tau increased the expression of E-cadherin, a marker of epithelial-mesenchymal transformation(EMT),and down-regulated the expression of N-cadherin, Vimentin and matrix metalloproteinases(MMPs),inhibited the AKT/GSK-3β pathway and reduced the β-catenin protein.Conclusion Tau can inhibit EMT,invasion and metastasis of cervical cancer cells, and the mechanism might be related to AKT/GSK-3β/β-catenin signaling pathway.更多还原