【摘要】 目的:检测丙酮酸脱氢酶蛋白X(PDHX)在胃癌组织中的表达水平，探索其与胃癌患者临床病理特征和预后的关系。方法:取经手术切除并由术后病理确诊的80例胃癌组织及相应癌旁组织构建胃癌组织芯片，通过免疫组织化学法检测PDHX在胃癌组织及癌旁组织中的表达情况，分析其与胃癌患者临床病理特征的关系。然后利用生物信息学技术分析PDHX与胃癌患者预后、免疫浸润相关分子的关系。结果:根据免疫组化结果，PDHX在癌旁组织中的表达明显高于胃癌组织；PDHX的表达与胃癌患者的年龄(P=0.002)、TNM分期(P=0.032)、肿瘤分化(P=0.001)、淋巴结转移(P=0.013)以及脉管侵犯(P=0.026)明显相关。胃癌患者中PDHX高表达者5年总体生存率及无进展生存率都高于低表达者。结论:PDHX在胃癌组织中的表达受到抑制且与患者预后相关，PDHX表达越高，患者预后越好，这表明PDHX可能成为胃癌治疗的潜在靶点。更多还原

【Abstract】 Objective:To investigate the expression and clinical prognosis of pyruvate dehydrogenase protein X(PDHX) in gastric cancer.Methods:Tissue microarray was constructed from 80 cases of gastric cancer and corresponding paracancerous tissues.The expression of PDHX in gastric cancer and paracancerous tissues was detected by immunohistochemical method, and the relationship between the expression and clinicopathological features of gastric cancer was analyzed.Then bioinformatics techniques were used to analyze the prognostic significance, immune infiltration and related molecular pathway analysis of PDHX.Results:According to the results of immunohistochemistry, the expression of PDHX in para cancerous tissues was significantly higher than that in gastric cancer tissues, and the expression of PDHX was significantly correlated with age(P=0.002),TNM stage(P=0.032),tumor differentiation(P=0.001),lymph node metastasis(P=0.013),and vascular invasion(P=0.026).The 5-year overall survival rate and progression-free survival rate of gastric cancer patients with high expression of PDHX were higher than those with low expression.Conclusion:The expression of PDHX in gastric cancer is inhibited and related to the prognosis of patients.The lower the expression of PDHX,the worse the prognosis, indicating that PDHX may be a potential target for the treatment of gastric cancer.更多还原