【摘要】 植物SHR蛋白参与调控植物生长发育中的多种代谢过程，尤其对根辐射形态的形成有很大影响。为了了解谷子中SHR基因在生长发育中的功能，参照拟南芥AtSHR基因序列，筛选并鉴定谷子中的SHR基因，并对其进行生物信息学分析，在此基础上对其在谷子中的表达模式进行分析。结果显示，谷子中有2个SHR基因，其中，SiSHR1位于9号染色体，CDS全长1 908 bp，编码635个氨基酸，SiSHR2位于2号染色体，CDS全长1 785 bp，编码594个氨基酸；2个SiSHR蛋白均为不稳定的酸性亲水蛋白；SiSHR蛋白的二级结构组成大部分为无规则卷曲与α-螺旋，有少部分β-折叠和延长链。SiSHR基因只含有1个外显子，与水稻的OsSHR亲缘关系更近，且均含有10个保守motif基序，SiSHR基因的启动子区含有光、激素（GA、ABA、生长素、水杨酸）等响应元件，SiSHR蛋白与CYCLIN、C2H2、SCL、SHBY、WOX蛋白互作，其中与SCL蛋白的互作方式为共表达；SiSHR基因在谷子中的表达模式呈时空特异性，SiSHR1/SiSHR2基因在谷子的根中有最高的表达，且在苗期的表达量高于灌浆期；SiSHR基因在谷子叶片中也有较高的相对表达量，且苗期表达量高于灌浆期。SiSHR基因可能通过应答GA、生长素和ABA信号途径，及蛋白互作的方式参与调控谷子叶片的发育及根的形态建成。更多还原

【Abstract】 Plant SHR proteins regulate various metabolic processes in plant growth and development, which has great influence on the formation of root radiation morphology. To understand the function of SHR genes in growth and development of Setaria italica, SHR genes in Setaria italic were screened and identified via reference of the sequence of AtSHR in Arabidopsis thaliana and was analyzed through bio-informatics software, based on work above, the expression pattern of the genes in Setaria italica was analyzed. The results showed that two SHR genes were identified in S. italica. Among them, SiSHR1 was located on Chr.9 with a CDS length of 1 908 bp, encoding 635 amino acids. SiSHR2 was located on Chr.2 with a CDS length of 1 785 bp,encoding 594 amino acids. The two SiSHR proteins were unstable and hydrophilic. The secondary structure of SiSHR proteins consisted mostly of random curl and α-helix, and a few β-folds and extended chain. SiSHR genes contained only one exon and 10conserved motifs, which was more closely related to OsSHR in Oryza sativa. The promoter region of SiSHR genes contained light, hormones(GA, ABA, auxin, salicylic acid) and other response elements. SiSHR proteins were predicted to interact with CYCLIN, C2H2, SCL, SHBY and WOX proteins and were co-expressed with SCL. The expression pattern of SiSHR genes were time-space specific. SiSHR1/2 genes had the highest expression in roots and the expression level in seedling stage was higher than that in grain filling stage. SiSHR genes also had higher relative expression in leaves and the expression level in seedling stage was higher than that in grain filling stage. In conclusion, SiSHR genes were probably involved in regulating the development of leaves and the morphogenesis of roots in S. italica by responding to GA, auxin and ABA signals and protein interactions.更多还原