【摘要】 目的：探讨苍耳子提取物抗类过敏作用及筛选出其中的活性成分。方法：采用测量小鼠脚掌肿胀和组织液渗出，检测小鼠血清中组胺、肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、单核细胞趋化因子-1(monocyte chemotactic protein-1,MCP-1)和白细胞介素-8(interleukin-8,IL-8)浓度，检测肥大细胞脱颗粒的方法，观察苍耳子提取物拮抗C48/80诱发的类过敏反应的作用。建立高表达Mrg X2/CMC筛选模型，从苍耳子的10种成分中筛选可作用于Mrg X2受体的抗类过敏活性成分。结果：苍耳子提取物可减轻C48/80导致的小鼠脚掌肿胀和组织液渗出，降低小鼠血清中组胺、TNF-α、MCP-1和IL-8的含量，抑制肥大细胞脱颗粒（P<0.05）。10种苍耳子成分中筛选出6种有保留成分，其中槲皮素和大黄素可显著抑制肥大细胞释放组胺（P<0.05）。结论：苍耳子提取物可抑制肥大细胞脱颗粒和致敏介质的释放，其成分中槲皮素和大黄素具有潜在的抗类过敏作用。更多还原

【Abstract】 Objective: The aim of this article is to investigate the anti-anaphylactoid effect of Fructus Xanthii extract and screen its active ingredients. Methods: The anti-effect of Fructus Xanthii extract on c48/80 induced anaphylactoid was investigated by measuring paw thickness increasing and tissue fluid exudation in mouse model, detecting concentration of histamine, tumor necrosis factor-α(TNF-α), monocyte chemotactic protein-1(MCP-1) and interleukin-8(IL-8) in mouse serum, detecting mast cells degranulation in LAD2cells. A Mrg X2 high-expression HEK293 cells membrane chromatography(Mrg X2/CMC) screening model was established, and the anti-anaphylactoid active ingredients that targeted on Mrg X2 receptors were screened from 10 ingredients of Fructus Xanthii. Results:Fructus Xanthii extract reduced mouse paw thickness increasing and tissue fluid exudation, reduced the release of histamine, TNF-α,MCP-1 and IL-8 in mouse serum, and inhibited the degranulation of LAD2 cells. There were 6 ingredients retained in the Mrg X2/CMC model from the 10 components of Xanthii fructus, among which Quercetin and emodin could significantly inhibit the release of histamine in LAD2 cells. Conclusions: Fructus Xanthii extract can inhibit the degranulation of mast cells and the release of allergic matter induced by C48/80, and quercetin and emodin from its ingredients have potential anti-anaphylactoid effects.更多还原