【摘要】 目的：研究不同浓度姜黄素（Cur）体外对胃癌SGC-7901细胞增殖、自噬性凋亡和TGF-β/Smad信号通路的影响。方法：体外培养SGC-7901细胞，以不同浓度Cur作用于SGC-7901细胞。MTT法检测不同浓度的Cur对SGC-7901细胞增殖的影响。Hoechst 33258法观察不同浓度Cur对SGC-7901细胞凋亡影响，流式细胞仪检测细胞凋亡率。划痕实验检测不同浓度Cur对SGC-7901迁移能力的影响。免疫印迹法检测细胞凋亡相关蛋白NF-κB、自噬相关蛋白Beclin1、LC3Ⅱ及TGF-β/Smad信号通路蛋白TGF-β和p-smad2/3表达。结果：Cur能够抑制胃癌SGC-7901细胞的增殖和迁移，并且Cur对增殖和迁移的影响具有浓度依赖性。Cur能够促进胃癌SGC-7901细胞自噬性凋亡的发生，Cur浓度越高，SGC-7901细胞凋亡率越高（P<0.05）。Cur处理胃癌SGC-7901细胞后NF-κB、Beclin1、LC3Ⅱ表达明显升高，而TGF-β、p-smad2/3表达明显降低，且NF-κB、Beclin1、LC3Ⅱ、TGF-β和p-smad2/3的变化具有浓度依赖性。结论：Cur能够抑制胃癌SGC-7901细胞增殖和迁移并诱导自噬性凋亡的发生，其机制与促进NF-κB、Beclin1、LC3Ⅱ表达，抑制TGF-β/Smad信号通路激活有关。更多还原

【Abstract】 Objective: To study the effects of different concentrations of curcumin(Cur) on the proliferation, autophagic apoptosis and TGF-β/Smad signaling pathway of gastric cancer SGC-7901 cells in vitro. Methods: SGC-7901 cells were cultured in vitro, and SGC-7901 cells were treated with different concentrations of Cur. The MTT method was used to detect the effect of different concentrations of Cur on the proliferation of SGC-7901 cells. The Hoechst 33258 method was used to observe the effect of Cur on the apoptosis of SGC-7901 cells. Flow cytometry was used to detect the apoptosis rate. The effect of different concentration of Cur on the migration ability of SGC-7901 was detected by scratch test. The apoptosis-related protein NF-κB, autophagy-related protein Beclin1, LC3 Ⅱ and TGF-β/Smad signaling pathway proteins TGF-β and p-smad2/3 expression were detected by Western blotting. Results: Cur could inhibit the proliferation and migration of gastric cancer SGC-7901 cells, and the effect of Cur on the proliferation and migration was concentration-dependent. Cur could promote the occurrence of autophagic apoptosis of gastric cancer SGC-7901 cells, the higher the Cur concentration, the higher the apoptosis rate of SGC-7901 cells(P<0.05). After Cur treatment of SGC-7901 cells, the expression of NF-κB, Beclin1, LC3Ⅱ were significantly increased, while the expression of TGF-β, p-smad2/3 was significantly decreased, and the expression of NF-κB, Beclin1, LC3 Ⅱ, TGF-β and p-smad2/3 changes were concentration-dependent. Conclusion: Cur can inhibit the proliferation and migration of gastric cancer SGC-7901 cells and induce autophagic apoptosis, the mechanism may be that Cur could promote the expression of NF-κB, Beclin1, LC3Ⅱ and inhibit the activation of TGF-β/Smad signal pathway.更多还原