【摘要】 为探究水榆花楸中绿原酸、芦丁、金丝桃苷3种活性物质的最佳提取工艺，为其他化学成分提取提供参考依据，以水榆花楸叶片为原料，以表面活性剂十二烷基硫酸钠水溶液为溶剂，采用超声辅助提取法提取绿原酸、芦丁、金丝桃苷3种活性物质，对超声功率、超声时间、超声温度、液料比和提取溶剂质量分数进行单因素试验，根据试验结果设计Box-Behnken响应面法优化提取工艺并进行验证。结果表明，最佳提取工艺为超声功率405 W、超声时间40 min、超声温度60℃、液料比20∶1(mL/g)、提取溶剂质量分数2%,该条件下绿原酸、芦丁、金丝桃苷3种活性物质得率分别为2.13%±0.06%、0.54%±0.02%、0.19%±0.02%,与理论预测值2.14%、0.54%、0.19%一致。相比索氏提取法，超声辅助法提取水榆花楸中3种活性物质得率略有提高，优化所得提取工艺具有高稳定性、高得率等特点，应用前景较好。更多还原

【Abstract】 In order to study the extraction technology of chlorogenic acid, rutin and hyperin from the leaves of Sorbus alnifolia, and provide reference for the extraction of other chemical components, the leaves of Sorbus alnifolia were used as raw materials, and the surfactant sodium dodecyl sulfate aqueous solution was used as solvent to extract three active substances, chlorogenic acid, rutin and hyperin by ultrasonic assisted method. Based on five single factor experiments of ultrasonic power, ultrasonic time, ultrasonic temperature, liquid-material ratio and mass fraction of extraction solvent, the Box-Behnken response surface method was designed to optimize the extraction process and verified. Results showed that: the optimum process parameters were determined as follows: ultrasonic power 405 W, ultrasonic time 40 min, ultrasonic temperature 60 ℃, liquid-solid ratio 20∶1(mL/g), extraction solvent mass fraction 2%. The yields of chlorogenic acid, rutin and hyperin were 2.13%±0.06%, 0.54%±0.02% and 0.19%±0.02%, which was consistent with the theoretical predicted values of 2.14%, 0.54% and 0.19%. Compared with Soxhlet extraction, the yield of three active substances in Sorbus alnifolia was slightly improved. The optimized extraction process has the characteristics of high stability and high yield, and has a good application prospect.更多还原