【摘要】 西兰花芽是多种生物活性成分的良好来源,营养价值极高,近年来被广泛研究。该文对西兰花芽提取物的抗肿瘤活性及其潜在机制进行探讨。通过观察细胞形态学变化,发现西兰花芽提取物可使结肠癌Caco-2细胞和肝癌HepG2细胞密度下降,且细胞出现凋亡状态。细胞增殖抑制率结果表明西兰花芽提取物能够抑制两种肿瘤细胞增殖,且呈剂量依赖性,CaCl₂组对Caco-2和HepG2细胞半数抑制浓度分别为（0.030±0.002）、（0.043±0.004）mg/mL。通过流式细胞术检测凋亡率,发现西兰花芽提取物可使细胞凋亡比例达到82.95%。活性氧（reactive oxygen species,ROS）水平和线粒体膜电位检测结果表明,西兰花芽提取物可能通过诱导细胞产生ROS,降低线粒体膜电势,促进细胞凋亡,从而抑制Caco-2细胞增殖。更多还原

【Abstract】 Broccoli sprouts are a very nutritious source of many beneficial ingredients and have been widely studied in recent years. The anti-tumor activity and potential mechanism of broccoli sprout extract were investigated in this study. Medium and high concentrations of broccoli sprout extract could reduce the cell density of Caco-2 and HepG2 cells. Signs of apoptosis were evident in images. Broccoli sprout extract inhibited the proliferation of the proliferation of Caco-2 and HepG2 tumor cell lines in a dose-dependent manner, with a 50% inhibition concentration in the presence of CaCl₂（0.030 ± 0.002） mg/mL and（0.043 ± 0.004） mg/mL,respectively. The apoptosis rate detected by flow cytometry reached 82.95% in samples treated with the extract.Measurements of reactive oxygen species（ROS） and mitochondrial membrane potential showed that Caco-2 cells were sensitive to broccoli sprout extract, which could induce Caco-2 cell apoptosis by inducing the production of reactive oxygen species and reducing mitochondrial membrane potential.更多还原