【摘要】 为研究合生元制剂对小鼠抗氧化能力、细胞因子和肠道菌群的影响，随机将48只BALB/c小鼠分为对照组、低剂量组（L）、中剂量组（M）和高剂量组（H），并灌胃生理盐水和不同剂量合生元制剂，14 d后收集小鼠粪便进行菌落计数和16S rDNA高通量测序，测定小鼠肝脏、小肠组织中抗氧化指标，检测血清中细胞因子白介素-6(interleukin-6,IL-6)、白介素-1β(interleukin-1β,IL-1β)水平。结果表明：小肠和肝脏组织中，各个剂量组总超氧化物歧化酶（total superoxide dismutase,T-SOD）活性、谷胱甘肽过氧化物酶（glutathione peroxidase,GSH-Px）活性、谷胱甘肽（glutathione,GSH）含量上升，丙二醛（malondialdehyde,MDA）含量下降。H组IL-1β水平极显著降低（p<0.05）。灌胃14 d后，各剂量组与灌胃前相比，乳杆菌和双歧杆菌数量增加，16S rDNA测序表明给予合生元制剂可以增加Rikenella、Alistipes、Bifidobacterium和Lactobacillus等丰富度。灌胃上述合生元制剂可以提高小鼠抗氧化能力，降低细胞因子水平，促进乳杆菌和双歧杆菌繁殖，对肠道菌群具有调节作用。更多还原

【Abstract】 This study aimed to test the effects of synbiotics on the antioxidant capacity and intestinal flora of mice. Forty-eight male BALB/c mice were randomly assigned into the control(normal saline)group and lowdose(L),medium-dose(M),and high-dose(H)synbiotic groups and administrated with corresponding agents by gavage. After 14 days,the feces were collected for the characterization of intestinal flora changes by colony counting and 16S rDNA high-throughput sequencing. The antioxidant indexes in the liver and small intestine tissues and the levels of interleukin-6(IL-6)and interleukin-1β(IL-1β)in the serum were measured. In the small intestine and liver tissues,the activities of total superoxide dismutase(T-SOD),glutathione peroxidase(GSH-Px),and glutathione(glutathione,GSH)rose,and the malondialdehyde(MDA)content reduced after the administration of synbiotics. The level of IL-6 in group H declined(p<0.01). The number of lactobacilli and bifidobacteria in each synbiotics group increased after 14 days of administration. The 16S rDNA high-throughput sequencing results demonstrated that the administration of synbiotics increased the abundance of Rikenella,Alistipes,Bifidobacterium and Lactobacillus. The administration of the above-mentioned synbiotics could improve the antioxidant capacity,reduce the levels of inflammatory cytokines,increase the number of lactobacillus and bifidobacteriain the intestine,and regulate the intestinal flora.更多还原