【摘要】 建立UPLC法快速测定辣条中苯甲酸、山梨酸、糖精钠含量的分析方法。样品用80%的乙腈提取,正己烷脱脂萃取,经Oasis PRiME HLB小柱萃取净化,采用C18色谱柱,以10 mmol·L^-1乙酸铵+乙腈溶液为流动相进行洗脱,外标法定量。结果表明,各组分在1～200μg·mL^-1线性良好,r> 0.999,检出限均为3 mg·kg^-1,定量限均为10 mg·kg^-1;添加10 mg·kg^-1、20 mg·kg^-1、100 mg·kg^-1 3个浓度水平的平均回收率为91.1%～97.3%,RSD为1.67%～4.32%。建立的方法简单快速,准确可靠,适用于辣条中苯甲酸、山梨酸、糖精钠含量的测定。更多还原

【Abstract】 A method was established for the determination of benzoic acid,sorbic acid and saccharin sodium in hot strips by UPLC.The sample was extracted with 80% acetonitrile,extracted with n-hexane,purified by Oasis PRiME HLB column,eluted with 10 mmol·L^-1 ammonium acetate+acetonitrile solution on C₁₈ column,and quantified by external standard method.The results show that each component has a good linearity within the range of 1～200μgmL^-1,r²> 0.999,the detection limit is 3 mg·kg^-1,and the quantification limit is 10 mg·kg^-1;The average recoveries were 91.1%～97.3% and RSD was 1.67%～4.32% at 10 mg·kg^-1,20 mgk·g^-1 and 100 mg·kg^-1 concentration levels.The method is simple,rapid,accurate and reliable,which is suitable for the determination of benzoic acid,sorbic acid and saccharin sodium in hot strips.更多还原