【摘要】 利用乙醇浸提、酶解和微波辅助法提取龙须草根部中的总黄酮,采用二倍稀释法和药敏纸片法探究龙须草根部总黄酮对枯草芽孢杆菌、金黄色葡萄球菌和大肠杆菌的最低抑菌浓度,并探讨pH值、紫外线和常见金属离子对龙须草根部总黄酮抑菌效果的影响。结果表明,龙须草根部黄酮对枯草芽孢杆菌、金黄色葡萄球菌、大肠杆菌的最低抑菌浓度为2.425 mg·mL^-1、2.425 mg·mL^-1、4.850 mg·mL^-1;龙须草根部总黄酮在pH=5～7、紫外照射、金属离子存在的条件下均有较好的抑菌稳定性,并且大多数金属离子在一定程度上能提高根部总黄酮的抑菌效果。更多还原

【Abstract】 The total flavonoids in the root of rush were extracted using ethanol extraction,enzyme lysis and microwave assistance method.Double dilution and sensitive paper tablets were used to investigate the minimum antibacterial concentration of B.subtilis,S.aureus and E.coli,and to explore the effect of pH value,ultraviolet light and metal ions.The results showed that the minimum inhibitory concentration of flavonoids on B.subtilis,S.aureus and E.coli was 2.425 mg·mL^-1,2.425 mg·mL^-1,4.850 mg·mL^-1.Total flavonoids in rush root have good antibacterial stability under pH=5～7,UV irradiation and metal ions,and most metal ions can improve the antibacterial effect of root total flavonoids to a certain extent.更多还原