【摘要】 为深入了解婴儿时期人乳中复杂的脂质含量的动态变化，采用超高效液相色谱-三重四极杆串联飞行时间质谱及具有高采集命中率的顺序窗口采集所有理论质谱模式（sequential window acquisition of all theoretical massspectra,SWATH）对不同泌乳阶段人乳中的脂质进行鉴定及相对定量分析。结果显示，在3个泌乳阶段的人乳中共检测到229种脂质。根据“脂质代谢途径研究计划”中的分类，共检测到五大类物质：甘油酯类（glycerolipid,GL）主要包括24种甘油二酯和28种甘油三酯；甘油磷脂类（glycerophospholipid,GP）主要包括33种磷脂酰乙醇胺、21种磷脂酰胆碱、8种磷脂酰丝氨酸、13种磷脂酰甘油、10种磷脂酸和7种磷脂酰肌醇；鞘脂类（sphingolipid,SP）化合物共22种；甾醇脂类（sterol lipid,ST）化合物共10种；脂肪酸类及其衍生物（fatty acids,FAs）共4种。随着泌乳期的延长，GL、SP的相对含量逐渐增加，而GP、ST、FAs的相对含量逐渐下降。本研究实现了人乳中脂质的全面定性与相对定量分析，方法灵敏度高、精密度及稳定性良好，同时搭配SWATH采集模式，为科学模拟各阶段人乳及开发不同段龄婴幼儿奶粉的研发提供一定参考依据。更多还原

【Abstract】 In order to understand the dynamic changes of complex lipid contents in human milk during infancy, sequential window acquisition of all theoretical mass spectra(SWATH) with high acquisition hit rate assisted ultra-high performance liquid chromatography-triple quadrupole time of flight tandem mass spectrometry(UPLC-Triple-TOF-MS/MS) was used to identify and quantitatively analyze lipids in human milk at different lactation stages. The results showed that a total of 229 lipids were detected in human milk at the three lactation stages. According to the “Lipid Metabolism and Pathways Strategy”, five classes of lipids were identified: 1) glycerolipids(GLs) mainly including 24 diglycerides and 28 triglycerides;2) glycerophospholipids(GPs) including 33 phosphatidylethanolamines, 21 phosphatidylcholines, 8 phosphatidylserines, 13phosphatidylglycerols, 10 phosphatidic acids and 7 phosphatidylinositols; 3) 22 sphingolipids(SP); 4) 10 sterol lipids(ST);and 5) 4 fatty acids(FAs) and their derivatives. The relative contents of GL and SP increased gradually with lactation time,while the relative contents of GP, ST and FAs decreased gradually. This study achieved a comprehensive qualitative and relative quantitative analysis of lipids in human milk. The developed method is sensitive, precise and stable. It provides a basis for the development of infant food simulating human milk at different stages of lactation.更多还原