【摘要】 目的 研究酵解与水解食用菌多糖对脾胃损伤小鼠的影响,并比较二者对脾胃损伤小鼠的调节作用。方法 50只SPF级ICR小鼠,分成空白组、模型组、阳性对照组、水解食用菌多糖组和酵解食用菌多糖组,每组雌雄各半。采用高脂高糖加酒法建立脾胃损伤模型,测定小鼠脏器指数、血清中超氧化物歧化酶(superoxide dismutase, SOD)、丙二醛(malondialdehyde, MDA)、一氧化氮(nitric oxide, NO)、肿瘤坏血因子(tumor necrosis factor-α, TNF-α)、白介素-6 (interleukin-6, IL-6)、胃泌素(gastrin, GAS)、胃动素(motilin, MTL)、二胺氧化酶(diamine oxidase, DAO)、谷丙转氨酶(glutamic-pyruvic transaminase, AST)、谷草转氨酶(glutamic-pyruvic transaminase, ALT)、肌酐(creatinine, CRE)和尿素氮(urea nitrogen, BUN)水平。利用主成分分析提取主成分并对各组间小鼠进行评分。结果 相较于模型组,水解食用菌多糖与酵解食用菌多糖组GAS、SOD水平极显著上升(P<0.01), MTL水平呈显著与极显著上升(P<0.05与P<0.01), TNF-α、BUN、AST水平极显著降低(P<0.01),ALT水平显著降低(P<0.05), NO、IL-6、MDA、CRE、DAO水平呈显著或极显著降低(P<0.05或P<0.01)。主成分分析评分图显示,模型组与干预组小鼠间有明显分离,且与水解食用菌多糖组相比,酵解食用菌多糖组和模型组之间的分离度更高。结论 酵解食用菌多糖和水解食用菌多糖均可通过提升机体免疫力和抗氧化能力,改善脾胃损伤伴随的炎症反应及肝肾代谢功能,且酵解食用菌多糖对小鼠的脾胃损伤具有更优的调节作用。更多还原

【Abstract】 Objective To study the effects of glycolysis and hydrolysis of edible fungus polysaccharides on spleen and stomach injury mice, and compare their regulatory effects on spleen and stomach injury mice. Methods Fifty SPF ICR mice were divided into blank group, model group, positive control group, water-extracted mushroom polysaccharide group and glycolic mushroom polysaccharide group, with male and female in half in each group. The spleen and stomach injury model was established by the method of high fat, high sugar and wine, the viscera index,serum levels of superoxide dismutase(SOD), malondialdehyde(MDA), nitric oxide(NO), tumor necrosis factor-α(TNF-α), interleukin-6(IL-6), gastrin(GAS), motilin(MTL), diamine oxidase(DAO), glutamic-pyruvic transaminase(AST), glutamic-pyruvic transaminase(ALT) and creatinine(CRE) were determined and urea nitrogen(BUN) levels.Principal component analysis was used to extract principal components and score mice in each group. Results Compared with model group, the levels of GAS and SOD in the hydrolysis and fermentation groups were significantly increased(P<0.01), the MTL level was increased significantly and extremely significantly(P<0.05 and P<0.01), the levels of TNF-α, BUN and AST were significantly decreased(P<0.01), and the ALT level was significantly decreased(P<0.05), the levels of NO, IL-6, MDA, CRE and DAO were significantly decreased(P<0.05or P<0.01). The results of principal component analysis showed that there was significant separation between the model group and the intervention group. of mice, and the separation degree between the model group and the glycolysis group was higher than that of the hydrolysis group. Conclusion The glycolysis and hydrolysis of edible fungus polysaccharides can improve the immune and antioxidant capacity of the body, improve the inflammatory response and liver and kidney metabolic function associated with spleen and stomach injury, and the glycolysis of edible fungus polysaccharides has a better regulation effect on spleen and stomach injury in mice.更多还原