【摘要】 采用螺杆挤出机制备不同纳米氧化锌(ZnO)含量的ZnO/聚乳酸(PLA)/聚己二酸/对苯二甲酸丁二酯(PBAT)复合材料，在扫描电镜(SEM)下观察脆断微观形貌并测试其力学性能，用稀释平板涂布法研究其抗菌性能，用CCK-8细胞增殖测定法、免疫荧光染色技术研究了这种复合材料对小鼠成肌细胞(C2C12)的体外生物相容性影响。结果表明，ZnO/PLA/PBAT(2/70/30)复合材料具有较高的力学性能(18.6 MPa),ZnO/PLA/PBAT(5/70/30)复合材料对大肠杆菌的抗菌率达到(93.92±0.67)%。在CCK-8细胞增殖、免疫荧光两种实验研究中，ZnO/PLA/PBAT复合材料在纳米ZnO含量较低(≤2%)具有更好的体外生物相容性。更多还原

【Abstract】 The zinc oxide(ZnO)/polylactic acid(PLA)/poly terephthalate-adipate-butylene glycol ester(PBAT)composite materials with different contents of nano ZnO were prepared by screw extruder.Each was observed under scanning electronic microscope brittle fracture morphology, and was tested for mechanical properties.Antibacterial properties were studied by dilution-plate method.CCK-8 with cell proliferation assay and immunofluorescent staining investigated biocompatibility of such composite materials on mouse myoblasts(C2 C12)in vitro.The results show that the ZnO/PLA/PBAT(2/70/30)composite has higher mechanical properties(18.6 MPa),and the antibacterial rate of the ZnO/PLA/PBAT(5/70/30)composite against Escherichia coli could reach(93.92±0.67)%.The two experimental studies of CCK-8 cell proliferation and immunofluorescence indicate that the ZnO/PLA/PBAT composite materials have better in vitro biocompatibility with lower nano-ZnO contents(≤2%).更多还原