【摘要】 目的 研究固本平喘方纳米脂质体雾化吸入剂（简称“固本平喘吸入剂”）对哮喘豚鼠免疫和基因表达的影响。方法 将48只豚鼠分为正常对照组、哮喘模型组、中药对照组、固本平喘吸入剂小剂量组（简称“小剂量组”）、固本平喘吸入剂大剂量组（简称“大剂量组”）、西药对照组6组。除正常对照组外，其余各组采用豚鼠腹腔注射含10%卵蛋白的0.9%NaCl溶液方法造模。哮喘模型组、中药对照组、小剂量组、大剂量组、西药对照组分别吸入0.9%NaCl溶液、小青龙合剂、固本平喘吸入剂、地塞米松磷酸钠干预。采用ELISA法检测过敏相关免疫学指标[白介素-4(IL-4)、白介素-5(IL-5)、白介素-12(IL-12)、白介素-25(IL-25)、γ干扰素（IFN-γ）];RT-PCR技术检测信号转导和转录激活因子6(STAT6)、肿瘤坏死因子-α(TNF-α)表达；HE染色观察肺组织病理情况。结果 与正常对照组比较，哮喘模型组IL-4、IL-5、IL-25水平升高，IL-12、IFN-γ水平下降，差异有统计学意义（P<0.05）。与哮喘模型组比较，各药物组IL-4、IL-5水平下降，IL-12、IFN-γ水平升高，同时中药对照组、小剂量组、西药对照组IL-25水平下降，差异有统计学意义（P<0.05）。与正常对照组比较，哮喘模型组STAT6、TNF-α mRNA表达升高，差异有统计学意义（P<0.05）。与哮喘模型组及中药对照组比较，小剂量组及大剂量组STAT6、TNF-α mRNA的表达降低，差异有统计学意义（P<0.05）。与西药对照组比较，小剂量组STAT6、TNF-α mRNA的表达降低，大剂量组TNF-α mRNA的表达降低，差异有统计学意义（P<0.05）。肺组织病理显示：正常对照组由于雾化等因素影响存在肺动脉壁增厚和轻微炎症的情况；哮喘模型组的炎症情况最为严重，动脉壁增厚；中药对照组炎症情况减轻，伴有肉芽组织增生；西药对照组存在肺组织结构受到破坏的情况；小剂量组肺动脉壁增厚，细支气管腔内少量分泌物，3例出现轻度间质性肺炎和局部间质性肺炎；大剂量组肺动脉壁增厚，轻度间质性肺炎，2例出现局部肺组织破坏，伴肉芽组织增生。结论 固本平喘吸入剂对哮喘豚鼠的免疫学指标（IL-4、IL-5、IL-12、IL-25、IFN-γ）,STAT6、TNF-α mRNA表达及肺组织病理有一定改善作用。更多还原

【Abstract】 Objective Pingchuan Inhaler)on immunity and gene expression in asthmatic guinea pigs.Methods normal control group,asthma model group,traditional Chinese medicine(TCM)control group,Guben Pingchuan inhaler low-dose group(hereinafter referred to as low-dose group),Guben Pingchuan Inhaler high-dose group(hereinafter referred to as high-dose group),and western medicine control group. All the groups except for the normal group received an intraperitoneal injection of 10% ovalbumin in 0.9% NaCl solution to make the guinea pig model. Asthma model group,TCM control group,low-dose group,high-dose group,and western medicine control group inhaled 0.9% NaCl solution,Xiaoqinglong Mixture,nano-liposome aerosol inhaler of Guben Pingchuan Formula,and dexamethasone sodium phosphate,respectively. ELISA was used to detect allergy-related immunological indicators[interleukin-4(IL-4),interleukin-5(IL-5),interleukin-12(IL-12),interleukin-25(IL-25),(-)];-transducer and activator of transcription 6(STAT6) and tumor necrosis factor-α(TNF-α) expression;HE staining was used to observe lung histopathology.ResultsThe serum levels of IL-4,IL-5 and IL-25 in the asthma model group were higher and the levels of IL-12 and IFN-γ were lower than those in the normal control group,and the differences were statistically significant(P<0.05). The serum levels of IL-4 and IL-5 were lower and the serum levels of IL-12 and IFN-γ were higher in each drug intervention group than those in the asthma model group. Besides,the levels of IL-25 were lower in the TCM control group,low-dose group and western medicine control group than that in the asthma model group,and the differences were statistically significant(P<0.05). The expression of STAT6 and TNF-α mRNA was higher in the asthma model group than that in the normal control group,and the differences were statistically significant(P<0.05). The expression of STAT6 and TNF-α mRNA was lower in both the low-dose and high-dose groups than that in the asthma model group and the TCM control group,and the differences were statistically significant(P<0.05). The expression of STAT6 and TNF-αmRNA was lower in the low-dose group and the expression of TNF-α mRNA was lower in the high-dose group than that in the western medicine control group,and the differences were statistically significant(P<0.05). Lung histopathology showed:the normal control group presented thickened pulmonary artery walls and mild inflammation due to nebulization and other factors;the asthma model group presented the most severe inflammatory condition and thickened arterial walls;the TCM control group presented alleviated inflammatory condition and proliferation of granulation tissue;the western medicine control group presented the destruction of lung tissue structure;the low-dose group presented thickened pulmonary artery walls,a small amount of secretion in the lumen of fine bronchi and 3 cases of mild/localized interstitial pneumonia;and the high-dose group presented thickened pulmonary artery walls,mild interstitial pneumonia,2 cases of localized lung tissue destruction,and proliferation of granulation tissue.ConclusionGuben Pingchuan Inhaler showed some improvement in immunological indicators(IL-4,IL-5,IL-12,IL-25,IFN-γ),expression of STAT6 and TNF-α mRNA,and lung histopathology in asthmatic guinea pigs.更多还原