【摘要】 目的 探讨血液样本56℃30 min加热灭活对精神类药物浓度监测的影响。方法 选取服用奥氮平、喹硫平、利培酮、锂盐、丙戊酸、氯氮平的患者各60例。将患者已完成药物浓度检测的剩余血液样本采用56℃30 min加热灭活后再次检测。对加热灭活前后各项药物检测结果的一致性进行分析。结果 样本加热灭活前后奥氮平、喹硫平、利培酮与9-羟利培酮之和、丙戊酸、氯氮平、N-去甲氯氮平的检测结果差异均无统计学意义(P>0.05),一致性较好。血锂加热灭活后的检测结果显著高于加热灭活前(P<0.05),加热灭活前后一致性较差。通过回归方程校准后,校准值与加热灭活前的检测结果差异无统计学意义(Z=-0.195,P=0.845),且一致性较好。结论 56℃30min加热灭活处理对奥氮平、喹硫平、利培酮与9-羟利培酮之和、丙戊酸、氯氮平、N-去甲氯氮平的检测无影响,但会影响血锂的检测。血锂结果通过回归方程校准后仍有临床价值。更多还原

【Abstract】 Objective To investigate the influence of heat inactivation of blood samples at 56 ℃ for 30 min on psychiatric drug concentration monitoring. Methods A total of 60 patients taking olanzapine,quetiapine,risperidone,lithium,valproic acid and clozapine were enrolled. The remaining blood samples were inactivated by heating at 56 ℃ for 30 min and then were determined again. The consistency of the results before and after heat inactivation was analyzed. Result The differences in the results of olanzapine,quetiapine,the sum of risperidone and 9-hydroxyrisperidone,valproic acid,clozapine and N-desmethylclozapine before and after heat inactivation of the samples were not statistically significant(P>0.05),which showed high consistency. The results of blood lithium after heat inactivation were higher than those before heat inactivation(P<0.05),and the consistency was poor before and after heat inactivation. After the calibration by regression equation,the differences between the calibration values and the assay results before heat inactivation were not statistically significant(Z=-0.195,P=0.845),which showed high consistency. Conclusions Heat inactivation at 56 ℃ for 30 min has no effect on the assay results of olanzapine,quetiapine,the sum of risperidone and 9-hydroxyrisperidone,valproic acid,clozapine and N-desmethylclozapine,but it affects the assay results of blood lithium. Blood lithium results still have the reference value after they are corrected by the regression equation.更多还原