【摘要】 目的：探究预电针心俞穴对急性心肌缺血模型大鼠心功能及穴区肥大细胞（mast cens,MC）活化的影响。方法：24只SD大鼠随机分成对照组、模型组和预电针组，每组8只。预电针组电针心俞穴，30 min/次，1次/d，共3 d,3 d后模型组和预电针组皮下注射盐酸异丙肾上腺素制备急性心肌缺血模型，对照组皮下注射生理盐水，记录各组大鼠不同状态心电图ST段偏移幅度；采用甲苯胺蓝染色检测右侧心俞穴区MC及脱颗粒MC数目。结果：预电针前后，3组大鼠心电图ST段偏移幅度比较差异无统计学意义（P>0.05）；造模后，与对照组比较，模型组ST段偏移幅度明显升高（P<0.01）,MC数目、脱颗粒率上升（P<0.01）；与模型组比较，预电针组ST段偏移幅度明显减小（P<0.01）,MC脱颗粒率降低（P<0.01）。结论：急性心肌缺血可造成大鼠右侧心俞穴呈现敏化态，电针预处理有预防心肌缺血的作用，可能与穴区MC募集及脱颗粒有关。更多还原

【Abstract】 Objective:To explore the effects of pre-electroacupuncture(EA)at Xinshu(BL15)on cardiac function and mast cells(MC)in the acupoint area of acute myocardial ischemia rats. Methods:24 SD rats were randomly divided into the control group,the model group and pre-EA group,with 8 rats per group. Rats in the pre-EA group were intervened with EA at bilateral Xinshu(BL15)for 30 minutes,once a day for three days. Acute myocardial ischemia model was prepared by subcutaneous injection of isoproterenol hydrochloride in the model and pre-electropriming groups after three days treatment. And the control group was injected with normal saline subcutaneously. ST segment deviation of electrocardiogram was recorded in each group of rats. Toluidine blue staining was used to detect the number of MC and degranulated MC in the right Xinshu point. Results:There was no significant difference in the ST segment deviation of electrocardiogram among 3 groups before and after electroacupuncture treatment(P >0.05);after modeling,the ST segment shift was significantly higher in the model group compared with the control group(P<0.01),and the number and degranulation rate of MCs increased(P <0.01);compared with the model group,the magnitude of ST segment shift was significantly reduced in the pre-EA group(P<0.01),and the MC degranulation rate was reduced(P<0.01). Conclusion:Acute myocardial ischemia can cause sensitization of the right Xinshu point in rats. Electroacupuncture preconditioning can prevent myocardial ischemia,which may be related to MC recruitment and degranulation in the acupoint area.更多还原