【摘要】 以豌豆分离蛋白为原料，采用酸性蛋白酶一次水解，水解条件为：加酶量5 000 U/g～6 000 U/g、酶解温度40℃～60℃，酶解时间90 min～120 min；采用NY50中性蛋白酶对酸性蛋白酶的水解液进行二次水解，对水解液进行膜分离，得到分子量300 Da～4 kDa的豌豆多肽，此多肽质量浓度在5 mg/mL～25 mg/mL范围时酪氨酸酶抑制率与多肽质量浓度呈量效关系。更多还原

【Abstract】 Pea protein isolate was used as raw material, and the hydrolysis conditions were as follows: adding enzyme amount was 5 000 U/g～6 000 U/g, enzymolysis temperature was 40 ℃～60 ℃, enzymolysis time was 90 min～120min; NY50 neutral protease was used to hydrolyze the hydrolysate of acid protease, and the hydrolysate was separated by membrane to obtain pea polypeptide with molecular weight of 300 DA～4 kDa. The tyrosinase inhibition rate of this polypeptide was dose-dependent on the polypeptide concentration in the range of 5 mg/L～25 mg/L.更多还原