【摘要】 为提升《中国药典》中经典名方银翘散薄层色谱鉴别标准，利用不同极性试剂对银翘散待测样品进行系统提取，得到不同极性部位的供试品溶液，采用系列薄层色谱法对银翘散中10种单味药的供试品溶液、展开条件、显色方法进行系统的筛选，建立了适合银翘散中金银花、连翘、薄荷、牛蒡子、淡豆豉、荆芥穗、甘草等7种单味药鉴别的系列薄层鉴别方法，并且实现了一块薄层板、一次展开即可分别对牛蒡子与甘草、淡豆豉与荆芥穗进行共同鉴别。所建立的银翘散中7种单味药鉴别的系列薄层鉴别方法，只需对照药材即可对复方中的7种单味药进行鉴别，重复性好、可操作性强、检测成本低，为《中国药典》中银翘散薄层鉴别内容补充和质量标准提升提供了实验依据，也为其他银翘散成方制剂的薄层色谱鉴别提供了参考。更多还原

【Abstract】 To improve the thin layer chromatographic(TLC) identification standard of Yinqiao Powder in Chinese Pharmacopoeia, Yinqiao Powder samples were systematically extracted using different reagents to obtain different sample solutions. A series of TLC(sTLC) was used to systematically study the sample solutions, development conditions, and color development methods to identify 10 single herbs in Yinqiao Powder. Lonicerae Japonicae Flos, Forsythiae Fructus, Menthae Haplocalycis Herba, Arctii Fructus, Sojae Semen Praeparatum, Schizonepetae Spica, and Glycyrrhizae Radix Et Rhizoma were identified in Yinqiao Powder using sTLC. Arctii Fructus, Glycyrrhizae Radix Et Rhizoma, Sojae Semen Praeparatum, and Schizonepetae Spica can be dispersed on a thin-layer plate one time for common identification. In this study, the identifications of seven single herbs in Yinqiao Powder via sTLC can be used for identifying standard herbs. This approach shows good reproducibility, strong operability, and low detection cost. Furthermore, it can provide an experimental basis for the content supplement and quality standard improvement of Yinqiao Powder in Chinese Pharmacopoeia and facilitate a reference for the TLC identification of other Yinqiao Powder formulations.更多还原