【摘要】 将指数生长期的球等鞭金藻（Isochrysisg albana）H24和棕鞭藻（Ochrosphaero neopolitana）H32接种到1 000 m L三角烧瓶的2f培养基中，第8 d添加0 mmol/L（对照组）、0.5 mmol/L、1.5 mmol/L、2.5 mmol/L和5.0mmol/L的钨酸钠（Na₂WO₄），光强为100μmol·m^-2s^-1，连续光照、充气，水温25℃，盐度31下培养5 d，探讨不同浓度Na₂WO₄对2株海洋金藻的生长及脂质积累的影响。结果表明，Na₂WO₄浓度对2株金藻的细胞密度、叶绿素含量、叶绿素荧光参数Fv/Fm（光系统II最大光化学效率）、r ETR（相对电子传递速率）、q P（光化学猝灭）和脂肪酸组成均有显著影响（P<0.05）。Na₂WO₄浓度对球等鞭金藻的总脂含量有显著影响（P<0.05）。0 mmol/L及0.5mmol/L Na₂WO₄浓度组2株金藻的最终细胞密度最大。在2.5 mmol/L Na₂WO₄浓度组，球等鞭金藻Fv/Fm值最高（第4～5 d），在0 mmol/L和2.5 mmol/L Na₂WO₄浓度组棕鞭藻的Fv/Fm值最高（第1～2 d）。2种金藻的总脂含量随Na₂WO₄浓度升高而增加，在5.0mmol/L时达到最大值（分别为31.91%和25.55%），分别比对照组高38.21%和25.24%。在5.0 mmol/L Na₂WO₄时，球等鞭金藻的18∶1（n-9）（34.54%）、饱和脂肪酸（SFA）（37.59%）和单不饱和脂肪酸（MUFA）（42.65%）含量达最大值；对照组多不饱和脂肪酸（PUFA）含量最高（36.23%），之后随Na₂WO₄浓度增高呈下降趋势。棕鞭藻的16∶0（14.69%）和SFA（32.15%）含量在5 mmol/L达最大值，对照组18∶1（n-9）含量最高（37.33%）；二十碳五烯酸（EPA,20∶5（n-3））和PUFA含量随Na₂WO₄浓度升高先增加后降低，2.5 mmol/L时达最大值，分别为10.72%和31.96%。更多还原

【Abstract】 Two species in Chrysophyta Isochrysisg albana H24 and Ochrosphaero neopolitana H32 in the exponential growth phase were inoculated and cultured into 2f culture medium in a 1 000 m L Erlenmeyer flask,and sodium tungstate dihydrate （Na₂WO₄）was added into the flask at a dose of 0 mmol/L （control group）,0.5 mmol/L,1.5 mmol/L,2.5 mmol/L and 5.0 mmol/L on the 8th day at light intensity of 100μmol·m^-2s^-1,continuous light and aeration,at water temperature of 25℃,and a salinity of 31 for five days to investigate the effect of different Na₂WO₄concentrations on the growth and lipid accumulation of the two Chrysophyta algae.The results showed that the cell density,chlorophyll content,chlorophyll fluorescence parameters （Fv/Fm,maximal photochemical efficiency in PSII）,r ETR （relative electron transport rate）,q P （photochemical quenching） and fatty acid compositions of two Chrysophyta algae were significantly influenced by Na₂WO₄concentrations （P<0.05）and the total lipid content of I.galbana was significantly influenced by Na₂WO₄concentrations （P<0.05）,with the maximal final cell densities of the two Chrysophyta algae in mmol/L)and the low concentration of Na₂WO₄（0.5 mmol/L）group.The maximal Fv/Fm value was found in I.galbana at middle concentration of Na₂WO₄（2.5 mmol/L）（4～5 d）and the maximal Fv/Fm value in O.neopolitana was observed at 0 mmol/L and 2.5mmol/L Na₂WO₄（1～2 d）.The total lipid contents in the two Chrysophyta algae were shown to be increased with the increase in concentration of Na₂WO₄,with the maximum 31.91%and 25.55%at 5.0 mmol/L Na₂WO₄,and 38.21%and 25.24%higher than those at 0 mmol/L Na₂WO₄,respectively.The maximal contents of 18∶1 （n-9）（34.54%）,saturated fatty acid （SFA）（37.59%）and mono unsaturated fatty acid （MUFA）（42.65%） in I.galbana were found at 5.0 mmol/L Na₂WO₄.The contents of PUFA（polyunsaturated fatty acid）in I.galbana were decreased with the in crease in concentration of Na₂WO₄,and the maximum value（36.23%）appeared in control group.The maximal 16∶0 （14.69%）and SFA（32.15%）contents in O.neopolitana were found at 5.0mmol/L Na₂WO₄group,and the maximal 18∶1（n-9）content in O.neopolitana was 37.33%in 0 mmol/L Na₂WO₄group.The contents of EPA[20∶5（n-3）]and PUFA in O.neopolitana were shown to be increased first and then decreased with the elevated concentration of Na₂WO₄,with the maximum（10.72%and 31.96%,respectively）at 2.5 mmol/L Na₂WO₄.更多还原