【摘要】 桃糖转运蛋白基因PpTST2在液泡膜糖转运过程中具有重要作用。本文以桃品种‘2014-9-34’为材料，克隆PpTST2，并对其进行生物信息学分析及转基因苹果愈伤功能鉴定。结果显示，PpTST2的cDNA序列总长为2 220 bp，编码740个氨基酸，预测其编码蛋白质分子量为78.91 kDa，等电点为4.8，启动子序列分析发现其具有干旱胁迫、激素响应、光反应和糖响应等多种顺式作用元件，PpTST2基因的表达明显受ABA的诱导，在‘王林’愈伤组织中过表达PpTST2可以提高可溶性糖含量。酵母单杂实验表明转录因子Pp ABRE1能够激活PpTST2的表达。更多还原

【Abstract】 The sugar transporter gene PpTST2(Prupe.5G006300) played an important role in tonoplast sugar transport in peach(Prunus persica). We cloned PpTST2 gene from the peach variety ‘2014-9-34’ and performed the bio-information analyses of the PpTST2, and verified the functions of PpTST2 by transforming ’Orin’ calli(Malus domestica). The results showed that the full length of the cDNA sequence of PpTST2 was 2 220 bp, encoding 740 amino acids. The predicted molecular weight and isoelectric point of the PpTST2 was 78.91 kDa and 4.8, respectively. Analysis of the PpTST2 promoter sequence showed that it contained drought stress, hormone, light, and sugar response cis-elements. Additionally, experiment results showed that hormone ABA could significantly induce expression of the PpTST2 gene. Overexpression of PpTST2 in ’Orin’ calli increased the content of soluble sugar. Yeast single-hybrid experiments showed that the transcription factor PpABRE1 could activate the expression of PpTST2.更多还原