【摘要】 目的 分析乙肝表面抗原（HBsAg）阴性乙肝病毒DNA(HBV DNA)阳性标本的补充试验结果。方法 选择2013年至2021年间的献血者样本，用酶联免疫吸附试验（ELISA）法筛查，无反应性标本用核酸检测技术（NAT）方法检测，对HBV DNA检测呈反应性的部分样本再进行乙肝五项进行补充试验。结果 对498 279份ELISA阴性的样本进行NAT检测，共检出HBV DNA阳性886例，隐匿性乙肝（OBI）检出率为1.78‰，对其中300份样本进行乙肝五项补充试验，发现抗-HBc阳性27例，抗-HBe、抗-HBc两项阳性62例，HBsAg、抗-HBe、抗-HBc阳性11例，抗-HBs、抗-HBc阳性163例，抗-HBs阳性10例，五项全阴共有27例。结论 献血者中存在血清学阳性OBI和血清学阴性OBI，补充试验对指导献血者就医，提升血站服务具有重要意义。更多还原

【Abstract】 Objective To analyze the supplementary test results of samples with negative HBsAg and positive HBV DNA. Methods Blood donor samples from 2013 to 2021 were selected to detected by enzyme linked immunosorbent assay(ELISA).ELISA negative samples were tested by nucleic acid testing(NAT).HBV DNA positive samples were supplemented with five supplementary tests. Results Among 498279 negative ELISA samples,a total of 886 positive HBV DNA cases were detected by NAT,and the detection rate of occult HBV infection(OBI) was 1.78‰.The five supplementary tests were carried out on 300 samples of them.There were 27 cases with positive anti-HBc,62 cases with positive anti-HBe and anti-HBc,11 cases with positive HBsAg,anti-HBe and anti-HBc,163 cases with positive antiHBs and anti-HBc,10 cases with positive anti-HBs,and 27 cases with total negative. Conclusion Seropositive OBI exist in blood donors,supplementary test is of great significance for guiding blood donors to seek medical treatment and improving blood bank services.更多还原