【摘要】 为挖掘巨菌草根际土壤细菌对植物病原真菌的生防潜能和促生潜力，本研究通过梯度稀释方法分离得到一株巨菌草根际细菌，结合菌落形态、生理生化及16S rDNA进化树分析结果，菌株PT-140鉴定为产气肠杆菌(Enterobacter aerogenes)。平板对峙试验结果表明，该菌株对稻瘟病菌的抑菌率最大，为34.56%。该菌株对禾谷镰刀菌、香蕉枯萎病菌1号和香蕉枯萎病菌4号三种植物病原菌也具有一定的抑菌率。生物学特性试验结果表明，溶血α型、淀粉水解试验、明胶液化试验、蛋白酶试验均为阴性。促生潜力试验中，该菌株具有较好溶磷能力、产铁载体能力，固氮、解硅酸盐能力较弱。更多还原

【Abstract】 In order to screen out the rhizosphere bacteria which have the biocontrol potential and growth promoting characteristics.A rhizosphere bacteria fromJUJUNCAO named PT-140 with antagonistic activity against plant pathogenic fungi and growth promoting effects was obtained.This strain was identified as Enterobacter aerogenes utilizing the physiological and biochemical characteristics which combined the sequence of 16 S rDNA.The plate confrontation method was used to assay the antagonism of the strain against 4 plant pathogenic strains.The results indicated that the PT-140 strain had antagonism against all 4 tested plant pathogenic fungus in some degree, of which the inhibition rate against the Magnaporthe orisea was the highest, reaching34.56%,and had inhibitory effect on the other three plant pathogens tested including Fusarium graminearum,FocTR1 and FocTR4.The strain PT-140 can make the hemolysis test α type, amylohydrolysis, gelatin liquefaction test, and protease test is negative.It had strong ability of the dissolving phosphorus and iron carriers and the nitrogen-fixing and desilicate silicon and potassium dissolving weakly.更多还原