【摘要】 大气CO₂浓度和温度升高是未来气候变化的主要特征之一。为明确大豆（Glycine max）叶片响应大气CO₂浓度和温度升高的转录谱特征，本研究利用开顶式气室（open top chamber, OTC）模拟大气CO₂浓度和温度升高条件，对大豆鼓粒初期（R5）叶片进行RNA-Seq测序和分析。结果表明，纯净序列经筛选后共获得5 646个差异表达基因，对差异基因进行基因本体（Gene Ontology, GO）注释分析，发现注释的基因涉及46个功能组，其中关于细胞过程、细胞以及结合的富集程度较高。通过对KEGG （The Kyoto Encyclopedia of Genes and Genomes）代谢途径富集分析发现，大气CO₂浓度升高引起14个差异表达基因上调表达，这些差异基因主要参与大豆光合作用相关代谢途径，而大气CO₂浓度与温度同时升高导致17个差异表达基因中16个表现为上调，这些差异表达基因主要参与调控脂肪酸代谢途径；单一温度升高并未引起大豆叶片基因表达发生明显变化。本研究可为深入探讨大豆对未来气候变化的分子响应机制提供参考。更多还原

【Abstract】 Climate changes, feature as the increased temperature and concentration of CO₂in the atmosphere,substantially affect the agricultural production. Soybean（Glycine max） is an important oil crop, but little is known about the gene regulation of soybean leaves response to elevated CO₂and warming, which were important to gain insights of soybean adaption and feedback to climate change. In this study, an OTC（open top chamber） experiment was performed to simulate the conditions of elevated CO₂and warming. Soybean leaves at the full pod stage（R5） were collected for transcriptome analysis. The results showed that 5 646differentially expressed genes（DEGs） can be annotated as 44 functional terms by Gene Ontology（GO）analysis, among which, cell processes, cell and binding had higher enrichment degree. The Kyoto Encyclopedia of Genes and Genomes（KEGG） analysis found that the expression of 14 differentially expressed genes which were mainly related with the regulation of photosynthesis pathway increased under elevated carbon dioxide condition. However, the expression of 16 out of 17 differentially expressed genes which were mainly related with fatty acid metabolism pathway increased under elevated CO₂and warming conditions.Warming did not induce any significant changes of differentially expressed genes. The results would provide theoretical basis for further exploring the molecular mechanism of soybean response to future climate changes.更多还原