【摘要】 VQ蛋白是一类具有"FxxxVQxLTG"保守氨基酸序列的植物非特异性蛋白,由于其序列中含有2个高度保守的二肽缬氨酸(valine)和谷氨酰胺(glutamine),故称VQ蛋白。该家族在植物生长发育和胁迫响应中发挥着重要作用,但在马铃薯(Solanum tuberosum)中研究较少。为探讨马铃薯VQ家族功能,本研究采用生物信息学方法对StVQ基因家族成员的基本特征进行了分析,并使用qPCR方法验证了其对多种胁迫的响应情况。结果显示:StVQ家族共有37个成员,命名为:StVQ1～StVQ37,依次分布在马铃薯的12条染色体上,其中的34个成员序列中不含有内含子;依照序列特征可将该家族分为9个亚家族;多序列比对结果显示StVQ家族蛋白存在7种变异类型。种内共线性分析共发现了1对串联重复基因和7对片段重复基因,对蛋白网络预测分析推断其与StWRKY存在互作,RNA-seq数据则显示StVQ基因在马铃薯的不同组织表达有差异,而且在不同胁迫下的表达量变化也不同。进一步的qPCR和启动子分析证实这些基因能够响应冷、盐或干旱等多种非生物胁迫。本研究结果为更深层次的StVQ功能验证和作用机理分析提供参考。更多还原

【Abstract】 VQ protein is a kind of non-specific plant protein with the conserved amino acid sequence of "FxxxVQxLTG". It is named as the VQ protein because it contains two highly conserved dipeptides(valine and glutamine). This family plays an important role in plant growth and development and stress response, but little research has been done in potatoes(Solanum tuberosum). In order to explore the functions of potato VQ gene family, the StVQ gene family members were analyzed by bioinformatics method, and the responses to various stresses were verified by qPCR. The results showed that 37 StVQs were identified in potato genome,named as StVQ1～StVQ37, which were distributed on 12 chromosomes in order, and 34 of them did not contain introns. According to the sequence characteristics, the family can be divided into 9 subfamilies. Sequence alignment results showed that there were 7 variation types in the StVQ family proteins. A couple of tandem repeat genes and 7 pairs of segmental duplication genes were founded with intraspecific collinearity analysis method. The protein network prediction analysis indicated that the StVQ family proteins interacted with the StWRKY. The RNA-seq data suggested that StVQ genes expressions varied at different tissue, and variations of expression level were also distinct under different conditions of stress. The further experiments of qPCR and promoter analysis demonstrated that those genes could respond to the abiotic stress of cold, salinity,drought, etc. The results of this study provide a reference for further functional verification and mechanism analysis of StVQ.更多还原