【摘要】 【目的】筛选通过干热处理杀死黄瓜种带菌且不影响种子萌发力的最佳温度与时间。【方法】在60、70、80、90、100℃下，对黄瓜种子进行2、4、6、8、10、12 h干热处理，以未经干热处理的黄瓜种子为对照（CK），计算发芽势和发芽率，在PDA培养基和LB培养基中分别测定黄瓜真菌性和细菌性种带菌灭活率。【结果】干热处理超过80℃，黄瓜种子发芽率显著降低（P<0.05）。在PDA培养基中，70℃和80℃处理2、4、6、8、10、12 h，真菌性种带菌灭活率均显著高于CK(P<0.05),70℃干热处理4 h灭活率最高，为97.33%。在LB培养基中，70℃和80℃处理2、4、6、8、10、12 h，细菌性种带菌灭活率均显著高于CK(P<0.05),70℃干热处理4 h灭活率最高，为96.67%。【结论】在干热处理中，黄瓜种子正常萌发且种带菌灭活效果最佳的处理为70℃、4 h。更多还原

【Abstract】 【Objective】To determine the optimal temperature and time for dry-heat treatment to destroy fungi and bacteria mixed in cucumber seed without affecting seed germination.【Methods】Cucumber seeds were dried at 60,70,80,90,and 100 ℃for 2,4,6,8,10,and 12 h,with seeds without dry-heat treatment used as control(CK). The germination energy and germination rate were assessed. The inactivation rates of fungal and bacterial on cucumber seed were tested in PDA and LB medium.【Results】The germination rate of cucumber seeds decreased significantly(P<0.05)when the dry-heat treatment was over 80 ℃. In PDA medium,the inactivation rate of fungi was significantly higher than that of CK(P<0.05)after treatment at70 ℃ and 80 ℃ for 2,4,6,8,10,and 12 h,with the inactivation rate of 70 ℃ for 4 h being the highest,at 97.33%. In LB medium,after treatment at 70 ℃ and 80 ℃ for 2,4,6,8,10,and 12 h,the inactivation rate of bacteria was significantly higher than that of CK(P<0.05),with the inactivation rate of 70 ℃ for 4 h being the highest,at 96.67%.【Conclusion】Under dry-heat treatment,cucumber seeds germinated normally and the optimal treatment of best inactivation efficacy of fungi and bacteria was 70 ℃ for 4 h.更多还原