【摘要】 肠道微生物被认为是动物机体的共生体，在营养物质代谢、免疫调控等方面发挥重要作用.入侵生物斑点福寿螺(Pomacea maculata)严重威胁农业生产和生态系统功能.本研究利用高通量测序技术对11只雌、雄斑点福寿螺的肠道微生物多样性、结构和功能进行研究.结果表明，在门水平上，斑点福寿螺肠道内容物主要优势菌为变形菌门(Proteobacteria)(43.52%)和软壁菌门(Tenericutes)(13.82%);在属水平上，主要优势菌为气单胞菌属(Aeromonas)(15.66%)和支原体属(Mycoplasma)(7.26%).雌、雄斑点福寿螺肠道微生物多样性和群落结构没有显著性差异(P>0.05).在斑点福寿螺肠道内容物中检测到一些有益菌以及潜在致病菌.基于PICRUSt分析预测斑点福寿螺肠道菌群功能，共得到24种代谢功能，其中氨基酸运输和代谢、通用功能预测、信号转导、细胞壁/膜、能量产生和转换丰度较大.更多还原

【Abstract】 Intestine microbiome are considered as symbionts of animal bodies and play an important role in nutrient metabolism and immune regulation. Pomacea maculata is an invasive species that has caused serious damage to aquatic crop production and ecosystem functioning. In this study, high-throughput sequencing was used to analyze the intestinal microbial diversity, community structure and function of 11 female and male P.maculata. At the phylum level, Proteobacteria(43.52%)and Tenericutes(13.82%)were the dominant bacteria in the intestinal content microbiome of P.maculata. At the genus level, the dominant bacteria were Aeromonas(15.66%)and Mycoplasma(7.26%). No significant difference in intestinal microbial diversity and microbial community structure was found between male and female group(P>0.05). Some beneficial bacteria and potential pathogenic bacteria were found in the intestinal content microbiome of P.maculata. PICRUSt analysis was used to predict the intestinal microbiome functions of P.maculata,and 24 metabolic functions were predicted. The amino acid transport and metabolism, general function prediction only, transcription, cell wall/membrane/envelope biogenesis, energy production and conversion accounted for a large proportion.更多还原