【摘要】 [目的]本文旨在探究海滨雀稗S-腺苷甲硫氨酸（SAM）合成途径与耐盐性的关系，为解析海滨雀稗的耐盐机制奠定基础，同时为基于SAM途径的植物耐盐育种提供优异基因资源。[方法]克隆了海滨雀稗PvSAMS1（Paspalum vaginatum S-adenosylmethionine synthetase 1）基因并异源转化野生型拟南芥，分析其耐盐表型及生理指标；通过外源SAM处理野生型海滨雀稗，检测NaCl胁迫下的表型差异和离子含量。[结果]NaCl胁迫下，PvSAMS1基因过表达拟南芥株系表现出更好的生长和较低的电解质渗漏率，其相对含水量、SAM含量、K⁺含量、抗氧化酶活性均显著高于野生型拟南芥，而Na⁺含量显著低于野生型拟南芥；外源添加0.5 mmol·L^-1 SAM能显著缓解海滨雀稗在NaCl胁迫下的伤害，表现出更高的叶片含水量和较低的电解质渗漏率，同时能抑制Na⁺积累和促进K⁺吸收，其中在500 mmol·L^-1NaCl处理下，Na⁺在叶片和根部的抑制率分别达到11.6%和22.5%,K⁺在叶片和根部的增加率分别达到39%和14.5%。[结论]PvSAMS1能显著促进SAM合成，调控NaCl胁迫下的离子平衡和增强抗氧化酶活性，进而提高植物耐盐性。更多还原

【Abstract】 [Objectives]This study was aimed to explore the relationship between the synthetic pathway of seashore paspalum S-adenosylmethionine（SAM）and salt tolerance, to lay a foundation for the analysis of the salt tolerance mechanism in seashore paspalum and to provide excellent genetic resources for plant salt tolerance breeding on basis of SAM pathway. [Methods]Seashore paspalum PvSAMS1（Paspalum vaginatum S-adenosylmethionine synthetase 1）gene was seperated and heterologously transformed into Arabidopsis. The salt-tolerant phenotypes were observed and physiological indicators were detected. Then, seashore paspalum was treated with exogenous SAM and the phenotypic differences and ion content under NaCl stress were measured. [Results]Under NaCl stress, PvSAMS1 overexpressing Arabidopsis lines showed better growth and lower electrolyte leakage rate, with their relative water content, SAM content, K⁺ content, and antioxidant enzymes activity significantly higher than those of wild-type Arabidopsis,while their Na⁺ content significantly lower than the wild-type Arabidopsis. The addition of 0.5 mmol·L^-1 SAM could significantly alleviate the damage of seashore paspalum under NaCl stress, showing higher leaf water content and lower electrolyte leakage rate, inhibiting Na⁺ accumulation and promoting K⁺ absorption. Under the treatment of 500 mmol·L^-1 NaCl concentration, the inhibition rates of Na⁺in leaves and roots were 11.6% and 22.5% respectively, and the increase rates of K⁺ in leaves and roots were 39% and 14.5% respectively. [Conclusions]PvSAMS1 could improve plant salt tolerance by significantly promoting SAM synthesis, regulating ion balance and enhancing antioxidant enzyme activity under NaCl stress.更多还原